A rapid cleaning method for diatoms
Visualitza/Obre
Data de publicació
2019-07-18ISSN
0269-249X
Resum
We describe here a protocol for cleaning diatoms when time is short and the amount of
sample is very limited. Essentially, the method consists of drying material onto coverslips and
cleaning it directly in situ using nitric acid (or hydrogen peroxide), which is evaporated to
dryness. After washing twice or a few times with deionized water, the coverslips are ready for
mounting in resin for light microscopy as usual, or attachment to stubs for scanning electron
microscopy. Besides speed, the method has the advantage that it often preserves some
frustules intact or leaves their different elements (and stages of valve formation) closely
associated with each other. Examples where the method is especially advantageous are to
clean small aliquots of cultures for identification or to act as vouchers, or to explore diversity
of the most abundant species in natural material (e.g. periphyton). It is less suitable for counts
in ecological or palaeoecological studies. We tabulate the many other cleaning methods to
provide context for the new method described here.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
Anglès
Matèries (CDU)
574 - Ecologia general i biodiversitat
Pàgines
19
Publicat per
Taylor & Francis
Publicat a
Diatom Research
Citació
Trobajo, Rosa, Mann, David G. 2019. “A Rapid Cleaning Method For Diatoms”. Diatom Research 34 (2): 115-124. doi.org/10.1080/0269249X.2019.1637785.
Programa
Aigües Marines i Continentals
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