Skeletal muscle metabolomics and blood biochemistry analysis reveal metabolic changes associated with dietary amino acid supplementation in dairy calves
De Almeida, André M.
The efects of diferent amino acid (AA) supplementations of milk protein-based milk replacers in pre-ruminant calves from 3 days to 7 weeks of age were studied. Animals were divided into 4 groups: Ctrl) Control group fed with milk protein-based milk replacer without supplementation; GP) supplementation with 0.1% glycine and 0.3% proline; FY) supplementation with 0.2% phenylalanine and 0.2% tyrosine; MKT) supplementation with 0.62% lysine, 0.22% methionine and 0.61% threonine. For statistical analysis, t-test was used to compare AA-supplemented animals to the Ctrl group. At week 7, body weight and average daily gain (ADG) were measured and blood samples and skeletal muscle biopsies were taken. Blood biochemistry analytes related to energy metabolism were determined and it was shown that MKT group had higher serum creatinine and higher plasma concentration of three supplemented AAs as well as arginine compared with the Ctrl group. GP group had similar glycine/proline plasma concentration compared with the other groups while in FY group only plasma phenylalanine concentration was higher compared with Control. Although the AA supplementations in the GP and FY groups did not afect average daily gain and metabolic health profle from serum, the metabolome analysis from skeletal muscle biopsy revealed several diferences between the GP-FY groups and the Ctrl-MKT groups, suggesting a metabolic adaptation especially in GP and FY groups.
636 - Explotació i cria d'animals. Cria del bestiar i d'animals domèstics
Is part of
Yu, K., Matzapetakis, M., Valent, D., Saco, Y., De Almeida, A., Terré, M., & Bassols, A. (2018). Skeletal muscle metabolomics and blood biochemistry analysis reveal metabolic changes associated with dietary amino acid supplementation in dairy calves. Scientific Reports, 8(1). doi:10.1038/s41598-018-32241-4
Grant agreement number
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2015-68463-C2-1-P/ES/DETERMINACION DE LOS AMINOACIDOS LIMITANTES PARA EL CRECIMIENTO DE LOS TERNEROS AMAMANTADOS Y SU FUNCIONALIDAD/
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2015-68463-C2-2-P/ES/ESTUDIO MOLECULAR DE LOS EFECTOS DE LA SUPLEMENTACION DE AMINOACIDOS EN TERNEROS AMAMANTADOS MEDIANTE UNA APROXIMACION BIOQUIMICA Y PROTEOMICA/
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