Inactivation of African swine fever virus inoculated in liquid plasma by spray drying and storage for 14 days at 4˚C or 20˚C
View/Open
Author
Blázquez, Elena
Navarro, Núria
Rodríguez, Carmen
Ródenas, Jesús
Polo, Javier
Publication date
2023-08-22ISSN
1932-6203
Abstract
African swine fever virus (ASFV) is a dsDNA virus that can cause high mortality in pigs of all
ages. Spray-dried porcine plasma (SDPP) is a highly digestible ingredient used in feed
because it benefits performance, gut function and immunity. The objectives were to test if
the spray-drying (SD) conditions along with post-drying storage of product for 14 days can
inactivate ASFV inoculated in liquid plasma. Fresh liquid porcine plasma was inoculated
with ASFV (BA71V) to a final concentration of 105.18 ±0.08 TCID50/mL of liquid plasma. Triplicate 2-L samples of spiked plasma were SD in a lab drier set at an outlet temperature of
80˚C or 71˚C. The final dried samples were stored at 4˚C or 20˚C for 14 d. Liquid and SD
samples were analyzed for ASFV infectivity in two mirror 24-well plaques containing VERO
cells monolayers. Wells were inoculated with different dilutions of SDPP dissolved 1:9 in
PBS. One plaque was immediately frozen at -80˚C and the other was incubated at 37˚C for
3 d. Each dilution was replicated 9 times. After incubation both plaques were analyzed for
ASFV by qRT-PCR. Results indicated that the SD process inactivated between 3.2 to 4.2
Logs ASFV TCID50/mL and 2.53 to 2.75 Logs TCID50/mL when the outlet temperature were
80˚C and 71˚C respectively. All SD samples stored at 4˚C or 20˚C for 14 d were absent of
infectious ASFV. The combination of SD and post drying storage at both temperatures for
14 d was able to inactive >5.18 ±0.08 Log10 of ASFV inoculated in liquid porcine plasma,
demonstrating that the manufacturing process for SDPP can be considered safe regarding
ASFV.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
9
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Blázquez, Elena, Joan Pujols, Joaquím Segalés, Núria Navarro, Carmen Rodrı́guez, Jesús Ródenas, and Javier Polo. 2023. “Inactivation of African Swine Fever Virus Inoculated in Liquid Plasma by Spray Drying and Storage for 14 Days at 4°C or 20°C.” PLOS ONE 18 (8): e0290395. doi: 10.1371/journal.pone.0290395.
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/