Protection of Spanish Ibex (Capra pyrenaica) against Bluetongue Virus Serotypes 1 and 8 in a Subclinical Experimental Infection
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Author
Lorca-Oro, Cristina
García-Bocanegra, Ignacio
Mentaberre, Gregorio
Granados, José Enrique
Solanes, David
Fandos, Paulino
Galindo, Iván
Domingo, Mariano
Lavín, Santiago
López-Olvera, Jorge Ramón
Publication date
2012-05-30ISSN
1932-6203
Abstract
Many wild ruminants such as Spanish ibex (Capra pyrenaica) are susceptible to Bluetongue virus (BTV) infection, which
causes disease mainly in domestic sheep and cattle. Outbreaks involving either BTV serotypes 1 (BTV-1) and 8 (BTV-8) are
currently challenging Europe. Inclusion of wildlife vaccination among BTV control measures should be considered in certain
species. In the present study, four out of fifteen seronegative Spanish ibexes were immunized with a single dose of
inactivated vaccine against BTV-1, four against BTV-8 and seven ibexes were non vaccinated controls. Seven ibexes (four
vaccinated and three controls) were inoculated with each BTV serotype. Antibody and IFN-gamma responses were
evaluated until 28 days after inoculation (dpi). The vaccinated ibexes showed significant (P,0.05) neutralizing antibody
levels after vaccination compared to non vaccinated ibexes. The non vaccinated ibexes remained seronegative until
challenge and showed neutralizing antibodies from 7 dpi. BTV RNA was detected in the blood of non vaccinated ibexes
from 2 to the end of the study (28 dpi) and in target tissue samples obtained at necropsy (8 and 28 dpi). BTV-1 was
successfully isolated on cell culture from blood and target tissues of non vaccinated ibexes. Clinical signs were unapparent
and no gross lesions were found at necropsy. Our results show for the first time that Spanish ibex is susceptible and
asymptomatic to BTV infection and also that a single dose of vaccine prevents viraemia against BTV-1 and BTV-8 replication.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
9
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Lorca-Oró, Cristina, Joan Pujols, Ignacio García‐Bocanegra, Gregorio Mentaberre, José Enrique Granados, David Solanes, Paulino Fandós, et al. 2012. “Protection of Spanish Ibex (Capra Pyrenaica) Against Bluetongue Virus Serotypes 1 and 8 in a Subclinical Experimental Infection.” PloS One 7 (5): e36380. doi:10.1371/journal.pone.0036380
Grant agreement number
INIA/Programa Nacional de Proyectos de Investigación Fundamental/FAU2008-00019-C03-01/ES/Epidemiología, control y aspectos entomológicos de la lengua azul (BTV) en rumiantes silvestres en España/
Program
Sanitat Animal
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- ARTICLES CIENTÍFICS [2555]
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