Development and validation of direct PCR and quantitative PCR assays for the rapid, sensitive, and economical detection of porcine circovirus 3
Visualitza/Obre
Autor/a
Franzo, Giovanni
Legnardi, Matteo
Centelleghe, Cinzia
Tucciarone, Claudia M.
Cecchinato, Mattia
Cortey, Martí
Drigo, Michele
Data de publicació
2018-04-09ISSN
1040-6387
Resum
Since the identification of species Porcine circovirus 2, the relevance of genus Circovirus has increased given its impact on the swine industry. A new species (Porcine circovirus 3, PCV-3) has been detected in association with various clinical conditions. Consequently, there is an urgent need for reliable and widely accessible tests for both routine diagnostic and research purposes. We developed a direct PCR (requiring no DNA extraction) and a quantitative (q)PCR targeting the conserved rep gene to detect the PCV-3 genome. Test performance was assessed by testing 120 field samples within different matrices. Both methods were sensitive (detection of 10 viral genome/µL), specific, and repeatable. The substantially perfect agreement between the 2 assays strongly supports their high sensitivity and specificity. The low cost and short processing time of the direct PCR protocol, together with the reliable quantitative results provided by qPCR, support the establishment of common testing guidelines.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
Anglès
Matèries (CDU)
619 - Veterinària
Pàgines
25
Publicat per
SAGE Publications
Publicat a
Journal of Veterinary Diagnostic Investigation
Citació
Franzo, Giovanni, Matteo Legnardi, Cinzia Centelleghe, Claudia M. Tucciarone, Mattia Cecchinato, Martí Cortey, Joaquim Segalés, and Michele Drigo. “Development and Validation of Direct PCR and Quantitative PCR Assays for the Rapid, Sensitive, and Economical Detection of Porcine Circovirus 3.” Journal of Veterinary Diagnostic Investigation 30, no. 4 (July 2018): 538–44. doi:10.1177/1040638718770495.
Programa
Sanitat Animal
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