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Design strategies for positively charged endolysins: Insights into Artilysin development
(Biotechnology Advances, 2023-09-06)Endolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for the development of novel antibacterial agents. ... -
Detecting harmful algal blooms with isothermal molecular strategies
(Trends in Biotechnology, 2019-08-06)The use of isothermal nucleic acid amplification strategies to detect harmful algal blooms (HABs) is in its infancy. We describe recent advances in these systems and highlight the challenges for the ... -
Detecting harmful algal blooms with nucleic acid amplification-based biotechnological tools
(Science of The Total Environment, 2020-08-10)Harmful algal blooms (HABs) represent a growing threat to aquatic ecosystems and humans. Effective HAB management and mitigation efforts strongly rely on the availability of timely and in-situ tools for ... -
Detection and genetic characterization of enteric viruses in diarrhoea outbreaks from swine farms in Spain
(Porcine Health Management, 2023-06-22)Background The aim of this work was to study the prevalence and distribution of Porcine astrovirus (PAstV), Porcine kobuvirus (PKoV), Porcine torovirus (PToV), Mammalian orthoreovirus (MRV) and Porcine ... -
Detection and genotyping of Porcine circovirus 2 (PCV‐2) and detection of Porcine circovirus 3 (PCV‐3) in sera from fattening pigs of different European countries
(Transboundary and Emerging Diseases, 2020-05-01)PCV-2 is considered one of the most economically important viral agents in swine worldwide. Recently, PCV-3 has been found in pigs affected by different disorders and in healthy animals. The objective ... -
Detection and identification of five common internal grain insect pests by multiplex PCR
(Food Control, 2017-08-07)Consumer demands for better quality food have led to research on new tools aimed at early detection of insect pests in agro food industries. In these industries, internal grain feeders are the most ... -
Detection and monitoring of Drosophila suzukii in raspberry and cherry orchards with volatile organic compounds in the USA and Europe
(Scientific Reports, 2021-03-25)Spotted wing drosophila (SWD) causes significant economic loss in fruit crops to growers worldwide. There is immediate need for efficacious and selective monitoring tools that can detect infestations ... -
Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay
(Analytica Chimica Acta, 2018-07-28)Karlodinium is a dinoflagellate responsible for fish-killing events worldwide. In Alfacs Bay (NW Mediterranean Sea), the presence of two Karlodinium species (K. veneficum and K. armiger) with different ... -
Detection of azaspiracids in mussels using electrochemical immunosensors for fast screening in monitoring programs
(Sensors and Actuators B: Chemical, 2018-02-06)Given the widespread occurrence of azaspiracids (AZAs), it is clearly necessary to advance in simple and low-cost methods for the rapid detection of these marine toxins in order to protect seafood ... -
Detection of Beta-Lactam-Resistant Escherichia coli and Toxigenic Clostridioides difficile Strains in Wild Boars Foraging in an Anthropization Gradient
(Animals, 2021-05-28)Disease transmission among wild boars, domestic animals and humans is a public health concern, especially in areas with high wild boar densities. In this study, fecal samples of wild boars (n = 200) ... -
Detection of Ciguatoxins and Tetrodotoxins in Seafood with Biosensors and Other Smart Bioanalytical Systems
(Foods, 2023-05-18)The emergence of marine toxins such as ciguatoxins (CTXs) and tetrodotoxins (TTXs) in non-endemic regions may pose a serious food safety threat and public health concern if proper control measures are ... -
Detection of Gambierdiscus and Fukuyoa single cells using recombinase polymerase amplification combined with a sandwich hybridization assay
(Journal of Applied Phycology, 2021-04-10)Dinoflagellates of the genera Gambierdiscus and Fukuyoa are known to produce several bioactive compounds including the potent neurotoxic ciguatoxins (CTXs) which are able to accumulate in fish and through ... -
Detection of Haplosporidium pinnae from Pinna nobilis Faeces
(Journal of Marine Science and Engineering (JMSE), 2022-02-17)Pinna nobilis (Linnaeus, 1758) is the largest bivalve endemic to the Mediterranean. It is distributed in a wide range of coastal environments, including estuaries. Pinna nobilis has recently become a ... -
Detection of homozygous genotypes for a putatively lethal recessive mutation in the porcine argininosuccinate synthase 1 (ASS1) gene
(Animal Genetics, 2019-11-15)The sequencing of the pig genome revealed the existence of homozygous individuals for a nonsense mutation in the argininosuccinate synthase 1 (ASS1) gene (rs81212146, c.944T>A, L315X). Paradoxically, ... -
Detection of isothermally amplified ostreid herpesvirus 1 DNA in Pacific oyster (Crassostrea gigas) using a miniaturised electrochemical biosensor
(Talanta, 2019-09-02)Given the threat that ostreid herpesvirus 1 (OsHV-1) poses to shellfish aquaculture, the need for rapid, user-friendly and cost-effective methods to detect this marine pathogen and minimise its impact ... -
Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein
(Veterinary Immunology and Immunopathology, 2019-09-09)Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective ... -
Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
(Science of the Total Environment, 2019-06-28)Ostreopsis cf. ovata is a benthic microalga distributed in tropical and temperate regions worldwide which produces palytoxins (PlTXs). Herein, an electrochemical biosensor for the detection of this toxic ... -
Detection of Paralytic Shellfish Toxins in Mussels and Oysters Using the Qualitative Neogen Lateral-Flow Immunoassay: An Interlaboratory Study
(Journal of AOAC International, 2018-03-01)Paralytic shellfish toxins (PSTs) in bivalve molluscs represent a public health risk and are controlled via compliance with a regulatory limit of 0.8 mg saxitoxin (STX)⋅2HCl equivalents per kilogram of ... -
Detection of Porcine Circovirus 3 in Wildlife Species in Spain
(Pathogens, 2020-05-01)Porcine circovirus 3 (PCV-3) is the third member of the family Circoviridae, genus Circovirus, able to infect swine. A high prevalence of viral DNA has been recorded in wild boars. Recently, PCV-3 DNA ... -
Detection of SARS-CoV-2 in a cat owned by a COVID-19−affected patient in Spain
(Proceedings of the National Academy of Sciences (PNAS), 2020-09-18)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, is considered a zoonotic pathogen mainly transmitted human to human. Few reports indicate that pets may ...