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Quality comparison of recombinant soluble proteins and proteins solubilized from bacterial inclusion bodies
(Elsevier, 2022-09-20)
Recombinant protein production in bacteria is often accompanied by the formation of aggregates, known as inclusion bodies (IBs). Although several strategies have been developed to minimize protein ...
Design strategies for positively charged endolysins: Insights into Artilysin development
(Elsevier, 2023-09-06)
Endolysins are bacteriophage-encoded enzymes that can specifically degrade the peptidoglycan layer of bacterial cell wall, making them an attractive tool for the development of novel antibacterial agents. ...
Startup strategy for nitrogen removal via nitrite in a BAF system
(Springer, 2018-10-26)
A biological aerated filter (BAF) pilot plant consisting of two reactors (aerobic and anoxic one) was used to determine a strategy to remove nitrogen via nitrite. RNA/DNA analysis was performed to assess ...
Exploring the impact of the recombinant Escherichia coli strain on defensins antimicrobial activity: BL21 versus Origami strain
(BMC, 2022-05-09)
The growing emergence of microorganisms resistant to antibiotics has prompted the development of alternative antimicrobial therapies. Among them, the antimicrobial peptides produced by innate immunity, ...
The future of recombinant host defense peptides
(BMC, 2022-12-21)
The antimicrobial resistance crisis calls for the discovery and production of new antimicrobials. Host defense peptides (HDPs) are small proteins with potent antibacterial and immunomodulatory activities ...
Gut eukaryotic communities in pigs: diversity, composition and host genetics contribution
(BMC, 2020-05-07)
Background
The pig gut microbiome harbors thousands of species of archaea, bacteria, viruses and eukaryotes such as protists and fungi. However, since the majority of published studies have been focused ...
Feeding pasteurized waste milk to preweaned dairy calves changes fecal and upper respiratory tract microbiota
(Frontiers Media, 2019-06-06)
In the present study bacterial communities from both, the gastrointestinal and respiratory tract of pre-weaned dairy calves fed two different milk-feeding programs were characterized using 16S rRNA gene ...
Exploring the use of leucine zippers for the generation of a new class of inclusion bodies for pharma and biotechnological applications
(BMC, 2020-09-04)
Background
Inclusion bodies (IBs) are biologically active protein aggregates forming natural nanoparticles with a high stability and a slow-release behavior. Because of their nature, IBs have been ...
Lactiplantibacillus plantarum: a new example of inclusion body producing bacteria
(BMC, 2023-06-09)
Background
Lactic Acid Bacteria such as Lactococcus lactis, Latilactobacillus sakei (basonym: Lactobacillus sakei) and Lactiplantibacillus plantarum (basonym: Lactobacillus plantarum) have gained ...
Copy Number Variation on ABCC2-DNMBP Loci Affects the Diversity and Composition of the Fecal Microbiota in Pigs
(American Society for Microbiology, 2023-05-31)
Genetic variation in the pig genome partially modulates the composition of porcine gut microbial communities. Previous studies have been focused on the association between single nucleotide polymorphisms ...