Recombinant PrPSc shares structural features with brain-derived PrPSc: Insights from limited proteolysis
Sevillano, Alejandro M.
Elezgarai, Saioa R.
Erickson-Beltran, Melissa L.
Requena, Jesús R.
Very solid evidence suggests that the core of full length PrPSc is a 4-rung β-solenoid, and that individual PrPSc subunits stack to form amyloid fibers. We recently used limited proteolysis to map the β-strands and connecting loops that make up the PrPSc solenoid. Using high resolution SDS-PAGE followed by epitope analysis, and mass spectrometry, we identified positions ~116/118, 133–134, 141, 152–153, 162, 169 and 179 (murine numbering) as Proteinase K (PK) cleavage sites in PrPSc. Such sites likely define loops and/or borders of β-strands, helping us to predict the threading of the β-solenoid. We have now extended this approach to recombinant PrPSc (recPrPSc). The term recPrPSc refers to bona fide recombinant prions prepared by PMCA, exhibiting infectivity with attack rates of ~100%. Limited proteolysis of mouse and bank vole recPrPSc species yielded N-terminally truncated PK-resistant fragments similar to those seen in brain-derived PrPSc, albeit with varying relative yields. Along with these fragments, doubly N- and C-terminally truncated fragments, in particular ~89/97-152, were detected in some recPrPSc preparations; similar fragments are characteristic of atypical strains of brain-derived PrPSc. Our results suggest a shared architecture of recPrPSc and brain PrPSc prions. The observed differences, in particular the distinct yields of specific PK-resistant fragments, are likely due to differences in threading which result in the specific biochemical characteristics of recPrPSc. Furthermore, recombinant PrPSc offers exciting opportunities for structural studies unachievable with brain-derived PrPSc.
619 - Veterinària
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Sevillano, Alejandro M., Natalia Fernández-Borges, Neelam Younas, Fei Wang, Saioa R. Elezgarai, Susana Bravo, and Ester Vázquez-Fernández et al. 2018. "Recombinant Prpsc Shares Structural Features With Brain-Derived Prpsc: Insights From Limited Proteolysis". PLOS Pathogens 14 (1): e1006797. Public Library of Science (PLoS). doi:10.1371/journal.ppat.1006797.
Grant agreement number
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/BFU2013-48436-C2-1-P/ES/Decodificando los priones: elucidación de la base estructural de la infectividad de proteinas/
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-65046-C2-1-R/ES/Estudio de los mecanismos moleculares implicados en la diversidad y estabilidad de las cepas priónicas/
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