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dc.contributor.authorMorais, Sofia
dc.contributor.authorTorres, Miguel
dc.contributor.authorHontoria, Francisco
dc.contributor.authorMonroig, Óscar
dc.contributor.authorVaró, Inma
dc.contributor.authorAgulleiro, María José
dc.contributor.authorNavarro, Juan Carlos
dc.contributor.otherProducció Animalca
dc.date.accessioned2020-12-14T16:42:49Z
dc.date.available2020-12-14T16:42:49Z
dc.date.issued2020-05-15
dc.identifier.citationMorais, Sofia, Miguel Torres, Francisco Hontoria, Óscar Monroig, Inma Varó, María José Agulleiro, and Juan Carlos Navarro. 2020. "Molecular And Functional Characterization Of Elovl4 Genes In Sparus Aurata And Solea Senegalensis Pointing To A Critical Role In Very Long-Chain (>C24) Fatty Acid Synthesis During Early Neural Development Of Fish". International Journal Of Molecular Sciences 21 (10): 3514. doi:10.3390/ijms21103514.ca
dc.identifier.issn1661-6596ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1021
dc.description.abstractVery long-chain fatty acids (VLC-FA) play critical roles in neural tissues during the early development of vertebrates. However, studies on VLC-FA in fish are scarce. The biosynthesis of VLC-FA is mediated by elongation of very long-chain fatty acid 4 (Elovl4) proteins and, consequently, the complement and activity of these enzymes determines the capacity that a given species has for satisfying its physiological demands, in particular for the correct development of neurophysiological functions. The present study aimed to characterize and localize the expression of elovl4 genes from Sparus aurata and Solea senegalensis, as well as to determine the function of their encoded proteins. The results confirmed that both fish possess two distinct elovl4 genes, named elovl4a and elovl4b. Functional assays demonstrated that both Elovl4 isoforms had the capability to elongate long-chain (C20–24), both saturated (SFA) and polyunsaturated (PUFA), fatty acid precursors to VLC-FA. In spite of their overlapping activity, Elovl4a was more active in VLC-SFA elongation, while Elovl4b had a preponderant elongation activity towards n-3 PUFA substrates, particularly in S. aurata, being additionally the only isoform that is capable of elongating docosahexaenoic acid (DHA). A preferential expression of elovl4 genes was measured in neural tissues, being elovl4a and elovl4b mRNAs mostly found in brain and eyes, respectively.ca
dc.format.extent18ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofInternational Journal of Molecular Sciencesca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleMolecular and Functional Characterization of Elovl4 Genes in Sparus aurata and Solea senegalensis Pointing to a Critical Role in Very Long-Chain (>C24) Fatty Acid Synthesis during Early Neural Development of Fishca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2013-40986-R/ES/Acidos grasos poliinsaturados de cadena muy larga en peces: biosintesis e implicaciones durante fases tempranas de desarrollo en especies cultivadas/ca
dc.relation.projectIDMICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2011-23502/ES/Formulaciones alternativas de piensos para peces: efecto sobre el metabolismo lipidico y sobre los gastro y neuropeptidos que regulan la ingesta en larvas y juveniles/ca
dc.relation.projectIDEC/FP7/274184/EU/Gastrointestinal and neuropeptides regulating fook intake in fish in response to dietary lipid composition/GASTRONEUROPEPca
dc.subject.udc637ca
dc.identifier.doihttps://doi.org/10.3390/ijms21103514ca
dc.contributor.groupAqüiculturaca


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