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dc.contributor.authorMarimon, Neus
dc.contributor.authorEduardo, Iban
dc.contributor.authorLeón, Maela
dc.contributor.authorBerbegal, Mónica
dc.contributor.authorArmengol, Josep
dc.contributor.authorLuque, Jordi
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2020-12-15T11:06:26Z
dc.date.available2022-03-24T12:00:21Z
dc.date.issued2020-10-17
dc.identifier.citationMarimon, Neus, Iban Eduardo, Maela León, Mónica Berbegal, Josep Armengol, and Jordi Luque. 2020. "A Qpcr-Based Method For The Detection And Quantification Of The Peach Powdery Mildew (Podosphaera Pannosa) In Epidemiological Studies". European Journal Of Plant Pathology 158 (4): 1005-1016. doi:10.1007/s10658-020-02136-0.ca
dc.identifier.issn0929-1873ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1024
dc.description.abstractA qPCR-based method was developed to detect and quantify Podosphaera pannosa, the main causal agent of peach powdery mildew. A primer pair was designed to target part of the ITS region of the fungal ribosomal DNA, which proved to be highly specific and sensitive. A minimum of 2.81 pg µL− 1 of P. pannosa DNA and 6 conidia mL− 1 in artificially-prepared conidia suspensions were found to be the limit of detection. Moreover, a quantification of conidia placed on plastic tapes commonly used in volumetric air samplers was performed. Regression equations on conidia quantification obtained either from aqueous conidia suspensions or conidia placed on plastic tapes were similar. The protocol was further validated in field conditions by estimating the number of P. pannosa conidia obtained with an air sampler, by both microscopic and molecular quantification. Both techniques detected the peaks of conidia production during a 4-month sampling period, and a significant correlation (r = 0.772) was observed between both quantification methods. Additionally, the molecular method was applied to detect latent fungal inoculum in different plant parts of peach trees. The pathogen was detected mainly on the bark of affected twigs, and to a lesser extent, in foliar buds. The method developed here can be applied in the study of P. pannosa epidemiology and can help in improving the management of this pathogen through its early detection and quantification.ca
dc.format.extent34ca
dc.language.isoengca
dc.publisherSpringerca
dc.relation.ispartofEuropean Journal of Plant Pathologyca
dc.rightsCopyright © 2020, Koninklijke Nederlandse Planteziektenkundige Verenigingca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleA qPCR-based method for the detection and quantification of the peach powdery mildew (Podosphaera pannosa) in epidemiological studiesca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.relation.projectIDMINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RTA2013-00004-C03-01/ES/Estrategias de control de enfermedades fúngicas aéreas y de raíz en frutales mediterráneos, basadas en criterios epidemiológicos y en la utilización de micorrizas arbusculares/ca
dc.subject.udc632ca
dc.identifier.doihttps://doi.org/10.1007/s10658-020-02136-0ca
dc.contributor.groupProtecció Vegetal Sostenibleca
dc.contributor.groupGenòmica i Biotecnologiaca


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Copyright © 2020, Koninklijke Nederlandse Planteziektenkundige Vereniging
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/