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Self-assembled monolayer-based immunoassays for okadaic acid detection in seawater as monitoring tools
dc.contributor.author | Leonardo, Sandra | |
dc.contributor.author | Toldrà, Anna | |
dc.contributor.author | Rambla-Alegre, Maria | |
dc.contributor.author | Fernández-Tejedor, Margarita | |
dc.contributor.author | Andree, Karl B. | |
dc.contributor.author | Ferreres, Laura | |
dc.contributor.author | Campbell, Katrina | |
dc.contributor.author | Elliott, Christopher T. | |
dc.contributor.author | O'Sullivan, Ciara K. | |
dc.contributor.author | Pazos, Yolanda | |
dc.contributor.author | Diogène, Jorge | |
dc.contributor.author | Campàs, Mònica | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2018-12-04T13:35:33Z | |
dc.date.available | 2019-11-14T23:01:14Z | |
dc.date.issued | 2017-11-14 | |
dc.identifier.citation | Leonardo, Sandra, Anna Toldrà, Maria Rambla-Alegre, Margarita Fernández-Tejedor, Karl B. Andree, Laura Ferreres, and Katrina Campbell et al. 2018. "Self-Assembled Monolayer-Based Immunoassays For Okadaic Acid Detection In Seawater As Monitoring Tools". Marine Environmental Research 133: 6-14. Elsevier BV. doi:10.1016/j.marenvres.2017.11.004. | ca |
dc.identifier.issn | 0141-1136 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/103 | |
dc.description.abstract | Rapid and cost-effective methods to monitor the presence of diarrhetic shellfish poisoning (DSP) toxins in seawater samples in an easy and reliable manner are required to protect human health and avoid economic losses to shellfish industry. Immunoassays for the detection of okadaic acid (OA) and dinophysistoxin-1 and dinophysistoxin-2 are developed by immobilising OA on self-assembled monothiols or dithiols in an ordered and oriented way, providing an effective limit of detection of ∼1 ng OA equiv./mL seawater. The immunoassays are applied to the analysis of the particulate fraction of seawater samples from two Catalan harbours (NW Mediterranean) and samples collected periodically from the Galician Rias (E Atlantic), as well as a reference mussel sample. Results are in agreement with LC-MS/MS and the certified values. OA concentration in seawater correlates with Dinophysis cell abundance, with a 1–2 weeks lag. The immunoassays provide powerful high-throughput analytical methods potentially applicable as alternative monitoring tools. | ca |
dc.format.extent | 31 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Marine Environmental Research | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Self-assembled monolayer-based immunoassays for okadaic acid detection in seawater as monitoring tools | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2014-56024-C2-2-R/ES/MICROSISTEMAS PARA LA DETECCION RAPIDA, FIABLE Y RENTABLE DE MICROALGAS TOXICAS IN SITU Y A TIEMPO REAL/SEASENING | ca |
dc.relation.projectID | INIA/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RTA2013-00096-00-00/ES/Promoción de una Acuicultura de bivalvos y pesquerías sostenible y segura mediante modelización y caracterización del riesgo de toxinas emergentes/PROMAQUA | ca |
dc.subject.udc | 63 | ca |
dc.identifier.doi | https://doi.org/10.1016/j.marenvres.2017.11.004 | ca |
dc.contributor.group | Aigües Marines i Continentals | ca |
dc.contributor.group | Aqüicultura | ca |
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