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dc.contributor.authorOliveira, Catarina C.V.
dc.contributor.authorFatsini, Elvira
dc.contributor.authorFernández, Ignacio
dc.contributor.authorAnjos, Catarina
dc.contributor.authorChauvigné, François
dc.contributor.authorCerdà, Joan
dc.contributor.authorMjelle, Robin
dc.contributor.authorFernandes, Jorge M.O.
dc.contributor.authorCabrita, Elsa
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-01-19T11:33:49Z
dc.date.available2021-01-19T11:33:49Z
dc.date.issued2020-11-28
dc.identifier.citationOliveira, Catarina C. V., Elvira Fatsini, Ignacio Fernández, Catarina Anjos, François Chauvigné, Joan Cerdà, Robin Mjelle, Jorge M. O. Fernandes, and Elsa Cabrita. 2020. "Kisspeptin Influences The Reproductive Axis And Circulating Levels Of Micrornas In Senegalese Sole". International Journal Of Molecular Sciences 21 (23): 9051. doi:10.3390/ijms21239051.ca
dc.identifier.issn1661-6596ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1056
dc.description.abstractKisspeptin regulates puberty and reproduction onset, acting upstream of the brain–pituitary–gonad (HPG) axis. This study aimed to test a kisspeptin-based hormonal therapy on cultured Senegalese sole (G1) breeders, known to have reproductive dysfunctions. A single intramuscular injection of KISS2-10 decapeptide (250 µg/kg) was tested in females and males during the reproductive season, and gonad maturation, sperm motility, plasma levels of gonadotropins (Fsh and Lh) and sex steroids (11-ketotestosterone, testosterone and estradiol), as well as changes in small non-coding RNAs (sncRNAs) in plasma, were investigated. Fsh, Lh, and testosterone levels increased after kisspeptin injection in both sexes, while sperm analysis did not show differences between groups. Let7e, miR-199a-3p and miR-100-5p were differentially expressed in females, while miR-1-3p miRNA was up-regulated in kisspeptin-treated males. In silico prediction of mRNAs targeted by miRNAs revealed that kisspeptin treatment might affect paracellular transporters, regulate structural and functional polarity of cells, neural networks and intracellular trafficking in Senegalese sole females; also, DNA methylation and sphingolipid metabolism might be altered in kisspeptin-treated males. Results demonstrated that kisspeptin stimulated gonadotropin and testosterone secretion in both sexes and induced an unanticipated alteration of plasma miRNAs, opening new research venues to understand how this neuropeptide impacts in fish HPG axis.ca
dc.format.extent21ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofInternational Journal of Molecular Sciencesca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleKisspeptin influences the reproductive axis and circulating levels of microRNAs in Senegalese soleca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDEC/COST/CA16120/EU/European Epitranscriptomics Network/EPITRANca
dc.relation.projectIDEC/H2020/683210/EU/Innovative epigenetic markers for fish domestication/EPIFISHca
dc.relation.projectIDMINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2015-17103/ES/ /ca
dc.relation.projectIDMICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/PID2019-103868RB-I00/ES/Ingeniería de proteínas dirigidas a las vesículas: un nuevo enfoque biotecnológico para la criopreservación de oocitos y embriones de peces/CRYOYOLKca
dc.subject.udc637ca
dc.identifier.doihttps://doi.org/10.3390/ijms21239051ca
dc.contributor.groupAqüiculturaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/