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dc.contributor.authorArrieta-Villegas, Claudia
dc.contributor.authorVidal, Enric
dc.contributor.authorMartín, Maite
dc.contributor.authorVerdés, Judit
dc.contributor.authorMoll, Xavier
dc.contributor.authorEspada, Yvonne
dc.contributor.authorSingh, Mahavir
dc.contributor.authorVillarreal-Ramos, Bernardo
dc.contributor.authorDomingo, Mariano
dc.contributor.authorPérez de Val, Bernat
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-02-03T09:51:24Z
dc.date.available2021-02-03T09:51:24Z
dc.date.issued2020-12-10
dc.identifier.citationArrieta-Villegas, Claudia, Enric Vidal, Maite Martín, Judit Verdés, Xavier Moll, Yvonne Espada, Mahavir Singh, Bernardo Villarreal-Ramos, Mariano Domingo, and Bernat Pérez de Val. 2020. "Immunogenicity And Protection Against Mycobacterium Caprae Challenge In Goats Vaccinated With BCG And Revaccinated After One Year". Vaccines 8 (4): 751. doi:10.3390/vaccines8040751.ca
dc.identifier.issn2076-393Xca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1063
dc.description.abstractVaccination has been proposed as a supplementary tool for the control of tuberculosis in livestock. The long-term immunogenicity elicited by bacillus Calmette-Guerin (BCG) and the efficacy of revaccination were investigated in thirty goat kids distributed into three groups: unvaccinated controls, BCG (vaccinated at week 0) and BCG-BCG (vaccinated at weeks 0 and 56). Sixty-four weeks after the first vaccination, all animals were challenged with Mycobacterium caprae and examined post-mortem (pathology and bacterial load) at week 73. Antigen-specific interferon-gamma (IFN-γ) release was measured throughout the experiment. At week 59, peripheral blood mononuclear cells were stained for CD4, CD45RO and IFN-γ to determine the presence of antigen-specific cells secreting IFN-γ. The BCG-BCG group showed reductions in rectal temperatures, M. caprae DNA load in pulmonary lymph nodes (LN), the volume of lesions in pulmonary LN, mineralization in lungs, and higher weight gains compared to unvaccinated controls. IFN-γ responses were undetectable from 32 weeks after primary vaccination until revaccination, when the BCG-BCG group showed detectable IFN-γ production and a greater percentage of antigen-specific CD4+CD45RO+IFNγ+ and CD4-CD45RO+IFNγ+ cells compared to the BCG and control groups, which may be an indicator of the mechanisms of protection. Thus, re-vaccination of goats with BCG appears to prolong protection against infection with M. caprae.ca
dc.format.extent16ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofVaccinesca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleImmunogenicity and protection against Mycobacterium caprae challenge in goats vaccinated with BCG and revaccinated after one yearca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDINIA-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-0043-C02-01/ES/Estudio de vacunas, estrategias de vacunación y nuevas herramientas de lucha frente a la tuberculosis en la especie caprina: Estrategia DIVA/ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3390/vaccines8040751ca
dc.contributor.groupSanitat Animalca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/