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Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay
dc.contributor.author | Toldrà, Anna | |
dc.contributor.author | Jauset-Rubio, Míriam | |
dc.contributor.author | Andree, Karl B. | |
dc.contributor.author | Fernández-Tejedor, Margarita | |
dc.contributor.author | Diogène, Jorge | |
dc.contributor.author | Katakis, Ioanis | |
dc.contributor.author | O'Sullivan, Ciara K. | |
dc.contributor.author | Campàs, Mònica | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2018-12-11T11:40:47Z | |
dc.date.available | 2019-07-28T22:01:25Z | |
dc.date.issued | 2018-07-28 | |
dc.identifier.citation | Toldrà, Anna, Míriam Jauset-Rubio, Karl B. Andree, Margarita Fernández-Tejedor, Jorge Diogène, Ioanis Katakis, Ciara K. O'Sullivan, and Mònica Campàs. 2018. "Detection And Quantification Of The Toxic Marine Microalgae Karlodinium Veneficum And Karlodinium Armiger Using Recombinase Polymerase Amplification And Enzyme-Linked Oligonucleotide Assay". Analytica Chimica Acta 1039: 140-148. Elsevier BV. doi:10.1016/j.aca.2018.07.057. | ca |
dc.identifier.issn | 0003-2670 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/106 | |
dc.description.abstract | Karlodinium is a dinoflagellate responsible for fish-killing events worldwide. In Alfacs Bay (NW Mediterranean Sea), the presence of two Karlodinium species (K. veneficum and K. armiger) with different toxicities has been reported. This work presents a method that combines recombinase polymerase amplification (RPA) with an enzyme-linked oligonucleotide assay (ELONA) to identify, discriminate and quantify these two species. The system was characterised using synthetic DNA and genomic DNA, and the specificity was confirmed by cross-reactivity experiments. Calibration curves were constructed using 10-fold dilutions of cultured cells, attaining a limit of detection of around 50,000 cells/L, far below the Karlodinium spp. alert threshold (200,000 cells/L). Finally, the assay was applied to spiked seawater samples, showing an excellent correlation with the spiking levels and light microscopy counts. This approach is more rapid, specific and user-friendly than traditional microscopy techniques, and shows great promise for the surveillance and management of harmful algal blooms. | ca |
dc.format.extent | 19 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Analytica Chimica Acta | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Detection and quantification of the toxic marine microalgae Karlodinium veneficum and Karlodinium armiger using recombinase polymerase amplification and enzyme-linked oligonucleotide assay | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/DISPOSITIVOS BIOANALITICOS PARA LA DETECCION RAPIDA Y RENTABLE DE GAMBIERDISCUS Y CIGUATOXINAS/CIGUASENSING | ca |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2014-56024-C2-2-R/ES/MICROSISTEMAS PARA LA DETECCION RAPIDA, FIABLE Y RENTABLE DE MICROALGAS TOXICAS IN SITU Y A TIEMPO REAL/SEASENSING | ca |
dc.subject.udc | 63 | ca |
dc.identifier.doi | https://doi.org/10.1016/j.aca.2018.07.057 | ca |
dc.contributor.group | Aigües Marines i Continentals | ca |
dc.contributor.group | Aqüicultura | ca |
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