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dc.contributor.authorReyes-López, Felipe E.
dc.contributor.authorIbarz, Antoni
dc.contributor.authorOrdóñez-Grande, Borja
dc.contributor.authorVallejos-Vidal, Eva
dc.contributor.authorAndree, Karl B.
dc.contributor.authorBalasch, Joan Carles
dc.contributor.authorFernández-Alacid, Laura
dc.contributor.authorSanahuja, Ignasi
dc.contributor.authorSánchez-Nuño, Sergio
dc.contributor.authorFirmino, Joana P.
dc.contributor.authorPavez, Leonardo
dc.contributor.authorPolo, Javier
dc.contributor.authorTort, Lluis
dc.contributor.authorGisbert, Enric
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-02-04T15:39:46Z
dc.date.available2021-02-04T15:39:46Z
dc.date.issued2021-02-04
dc.identifier.citationReyes-López, Felipe E., Antoni Ibarz, Borja Ordóñez-Grande, Eva Vallejos-Vidal, Karl B. Andree, Joan Carles Balasch, and Laura Fernández-Alacid et al. 2021. "Skin Multi-Omics-Based Interactome Analysis: Integrating The Tissue And Mucus Exuded Layer For A Comprehensive Understanding Of The Teleost Mucosa Functionality As Model Of Study". Frontiers In Immunology 11. doi:10.3389/fimmu.2020.613824.ca
dc.identifier.issn1664-3224ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1081
dc.description.abstractFrom a general structural perspective, a mucosal tissue is constituted by two main matrices: the tissue and the secreted mucus. Jointly, they fulfill a wide range of functions including the protection of the epithelial layer. In this study, we simultaneously analyzed the epithelial tissue and the secreted mucus response using a holistic interactome-based multi-omics approach. The effect of the gilthead sea bream (Sparus aurata) skin mucosa to a dietary inclusion of spray-dried porcine plasma (SDPP) was evaluated. The epithelial skin microarrays-based transcriptome data showed 194 differentially expressed genes, meanwhile the exuded mucus proteome analysis 35 differentially synthesized proteins. Separately, the skin transcripteractome revealed an expression profile that favored biological mechanisms associated to gene expression, biogenesis, vesicle function, protein transport and localization to the membrane. Mucus proteome showed an enhanced protective role with putatively higher antioxidant and antimicrobial properties. The integrated skin mucosa multi-interactome analysis evidenced the interrelationship and synergy between the metabolism and the exuded mucus functions improving specifically the tissue development, innate defenses, and environment recognition. Histologically, the skin increased in thickness and in number of mucous cells. A positive impact on animal performance, growth and feed efficiency was also registered. Collectively, the results suggest an intimate crosstalk between skin tissue and its exuded mucus in response to the nutritional stimulus (SDPP supplementation) that favors the stimulation of cell protein turnover and the activation of the exudation machinery in the skin mucosa. Thus, the multi-omics-based interactome analysis provides a comprehensive understanding of the biological context of response that takes place in a mucosal tissue. In perspective, this strategy is applicable for evaluating the effect of any experimental variable on any mucosal tissue functionality, including the benefits this assessment may provide on the study of the mammalian mucosa.ca
dc.format.extent20ca
dc.language.isoengca
dc.publisherFrontiers Mediaca
dc.relation.ispartofFrontiers in Immunologyca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleSkin Multi-Omics-Based Interactome Analysis: Integrating the Tissue and Mucus Exuded Layer for a Comprehensive Understanding of the Teleost Mucosa Functionality as Model of Studyca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2014-51839-C5-5-R/ES/Fuentes proteicas y tratamientos alternativos para mejorar la sostenibilidad de los cultivos de dorada y lenguado/ca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-70637-R/ES/Caracterización funcional del mucus epidermico en peces de interés productivo: metodologia no invasiva de identificación de biomarcadores y relación con el estadio fisiológico/ca
dc.relation.projectIDMAPAMA/JACUMAR/ /ES/Estrategias nutricionales para la mejora en el rendimiento productivo: uso de piensos funcionales y dietas salud en acuicultura/DIETAplusca
dc.relation.projectIDMICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/117RT0521/ES/Estrategias de desarrollo y mejora de la producción de larvas de peces en Iberoamerica/LARVAplusca
dc.relation.projectIDEC/H2020/727315/EU/Mediterranean Aquaculture Integrated Development/MedAIDca
dc.subject.udc637ca
dc.identifier.doihttps://doi.org/10.3389/fimmu.2020.613824ca
dc.contributor.groupAqüiculturaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/