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dc.contributor.authorAuray, Gaël
dc.contributor.authorTalker, Stephanie C.
dc.contributor.authorKeller, Irene
dc.contributor.authorPython, Sylvie
dc.contributor.authorGerber, Markus
dc.contributor.authorLiniger, Matthias
dc.contributor.authorGanges, Llilianne
dc.contributor.authorBruggmann, Rémy
dc.contributor.authorRuggli, Nicolas
dc.contributor.authorSummerfield, Artur
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-02-04T15:54:17Z
dc.date.available2021-02-04T15:54:17Z
dc.date.issued2020-07-07
dc.identifier.citationAuray, Gaël, Stephanie C. Talker, Irene Keller, Sylvie Python, Markus Gerber, Matthias Liniger, Llilianne Ganges, Rémy Bruggmann, Nicolas Ruggli and Artur Summerfield. 2020. “High-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivo”. Front. Immunol. 11: 1429. doi: 10.3389/fimmu.2020.01429.ca
dc.identifier.issn1664-3224ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1082
dc.description.abstractThe present study investigated the transcriptomic response of porcine dendritic cells (DC) to innate stimulation in vitro and in vivo. The aim was to identify DC subset-specialization, suitable Toll-like receptor (TLR) ligands targeting plasmacytoid DC (pDC), and the DC activation profile during highly and low virulent classical swine fever virus (CSFV, strain Eystrup and Pinar del Rio, respectively) infection, chosen as model for a virus causing a severe immunopathology. After identification of porcine conventional DC (cDC) 1, cDC2, pDC and a monocyte-derived subset in lymphoid tissues, we characterized DC activation using transcriptomics, and focused on chemokines, interferons, cytokines, as well as on co-stimulatory and inhibitory molecules. We demonstrate that porcine pDC provide important signals for Th1 and interferon responses, with CpG triggering the strongest responses in pDC. DC isolated early after infection of pigs with either of the two CSFV strains showed prominent upregulation of CCL5, CXCL9, CXCL10, CXCL11, and XCL1, as well as of the cytokines TNFSF13B, IL6, IL7, IL12B, IL15, IL27. Transcription of IL12B and many interferon genes were mostly restricted to pDC. Interestingly, the infection was associated with a prominent induction of inhibitory and cell death receptors. When comparing low and highly virulent CSFV strains, the latter induced a stronger inflammatory and antiviral response but a weaker cell cycle response, and reduced antigen presentation functions of DC. Taken together, we provide high-resolution information on DC activation in pigs, as well as information on how DC modulation could be linked to CSFV immunopathology.ca
dc.format.extent22ca
dc.language.isoengca
dc.publisherFrontiers Mediaca
dc.relation.ispartofFrontiers in Immunologyca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleHigh-Resolution Profiling of Innate Immune Responses by Porcine Dendritic Cell Subsets in vitro and in vivoca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3389/fimmu.2020.01429ca
dc.contributor.groupSanitat Animalca


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