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dc.contributor.authorTaranto, Francesca
dc.contributor.authorD’Agostino, Nunzio
dc.contributor.authorCatellani, Marcello
dc.contributor.authorLaviano, Luca
dc.contributor.authorRonga, Domenico
dc.contributor.authorMilc, Justyna
dc.contributor.authorPrandi, Barbara
dc.contributor.authorBoukid, Fatma
dc.contributor.authorSforza, Stefano
dc.contributor.authorGraziano, Sara
dc.contributor.authorGullì, Mariolina
dc.contributor.authorVisioli, Giovanna
dc.contributor.authorMarmiroli, Nelson
dc.contributor.authorBadeck, Franz-W.
dc.contributor.authorMinervini, Anna Paola
dc.contributor.authorPecorella, Ivano
dc.contributor.authorPecchioni, Nicola
dc.contributor.authorDe Vita, Pasquale
dc.contributor.authorFrancia, Enrico
dc.contributor.otherIndústries Alimentàriesca
dc.date.accessioned2021-02-24T15:08:18Z
dc.date.available2021-02-24T15:08:18Z
dc.date.issued2020-08-20
dc.identifier.citationTaranto, Francesca, Nunzio D’Agostino, Marcello Catellani, Luca Laviano, Domenico Ronga, Justyna Milc, and Barbara Prandi et al. 2020. "Characterization Of Celiac Disease-Related Epitopes And Gluten Fractions, And Identification Of Associated Loci In Durum Wheat". Agronomy 10 (9): 1231. doi:10.3390/agronomy10091231.ca
dc.identifier.issn2073-4395ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1148
dc.description.abstractWhile durum wheat is a major food source in Mediterranean countries, storage (i.e., gluten) proteins are however responsible for celiac disease (CD), a serious autoimmune disease that occurs in genetically predisposed subjects. Different gluten epitopes—defined as “immunogenic” (IP) and “toxic” (TP) peptides—are involved in the pathology and their content in wheat grain depends on environmental and genetic factors. Detection of IP and TP is not trivial, and no work has been conducted so far to identify the genomic regions associated with their accumulation in wheat. In the present study, a genome-wide association study was performed on a durum wheat collection to identify marker–trait associations (MTAs) between 5730 high quality SNPs and the accumulation of CD-related peptides and gluten protein composition measured in two consecutive cropping seasons (2015/2016 and 2016/2017). High-molecular-weight glutenin subunits (HMW-GS) were more stable between the two years, and differences in total gluten proteins were mainly due to low-molecular-weight glutenin subunits (LMW-GS) and accumulation of gliadins. In the first instance, association tests were conducted on yellow pigment content (YP), a highly inheritable trait with a well-known genetic basis, and several significant MTAs were found corresponding to loci already known for being related to YP. These findings showed that MTAs found for the rest of the measured traits were reliable. In total, 28 significant MTAs were found for gluten composition, while 14 were found to be associated with IP and TP. Noteworthy, neither significant (−log10p > 4.7) nor suggestive (−log10p > 3.3) MTAs for the accumulation of CD-triggering epitopes were found on Gli-A1/Glu-A3 and Gli-B1/Glu-B3 loci, thus suggesting regulatory rather than structural gene effect. A PBF transcription factor on chromosome 5B, known to be involved in the regulation of the expression of CD-related peptides, was identified among the positional candidate genes in the LD-decay range around significant SNPs. Results obtained in the present study provide useful insights and resources for the long-term objective of selecting low-toxic durum wheat varieties while maintaining satisfactory gluten quality.ca
dc.format.extent22ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofAgronomyca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleCharacterization of Celiac Disease-Related Epitopes and Gluten Fractions, and Identification of Associated Loci in Durum Wheatca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc663/664ca
dc.identifier.doihttps://doi.org/10.3390/agronomy10091231ca
dc.contributor.groupSeguretat Alimentàriaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/