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dc.contributor.authorDíaz, Ivan
dc.contributor.authorPujols, Joan
dc.contributor.authorCano, Esmeralda
dc.contributor.authorCortey, Martí
dc.contributor.authorNavarro, Núria
dc.contributor.authorVidal, Anna
dc.contributor.authorMateu, Enric
dc.contributor.authorMartín, Marga
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-03-11T15:52:36Z
dc.date.issued2021-03-04
dc.identifier.citationDíaz, Ivan, Joan Pujols, Esmeralda Cano, Martí Cortey, Núria Navarro, Anna Vidal, Enric Mateu, and Marga Martín. 2021. "The Immune Response Does Not Prevent Homologous Porcine Epidemic Diarrhoea Virus Reinfection Five Months After The Initial Challenge.". Transboundary And Emerging Diseases. doi:10.1111/tbed.14055.ca
dc.identifier.issn1865-1674ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1186
dc.description.abstractThe aim of the present study was to evaluate the duration of protective immunity against Porcine epidemic diarrhoea virus (PEDV). To do so, a two phases study was performed. In the first phase, 75 four‐week‐old pigs (group A) were orally inoculated (0 days post‐inoculation; dpi) with a European PEDV G1b strain and 14 were kept as controls (group B). The second phase started five month later (154 dpi), when animals in group A were homologous challenged and animals in group B were challenged for first time. Clinical signs, viral shedding and immune responses were evaluated after each inoculation, including the determination of antibodies (ELISA and viral neutralisation test, IgA and IgG ELISPOTs using peripheral blood mononuclear cells and lymph node cells) and the frequency of interferon‐gamma (IFN‐γ) secreting cells. During the first phase, loose stools/liquid faeces were observed in all group A animals. Faecal shedding of PEDV occurred mostly during the first 14 days but, in some animals, persisted until 42 dpi. All inoculated animals seroconverted for specific‐PEDV IgG and IgA, and for neutralizing antibodies (NA). At 154 dpi, 77% of pigs were still positive for NA. After that, the homologous challenge resulted in a booster for IgG, IgA, NA, as well as specific‐PEDV IgG, IgA and IFN‐γ secreting cells. In spite of that, PEDV was detected in faeces of all pigs from group A, indicating that the immune response did not prevent reinfection, although the duration of the viral shedding and the total load of virus shed was significantly lower for previously challenged pigs (p<0.05). Taken together, the results indicated that, potentially, maintenance of PEDV infection within an endemic farm may occur by transmission to and from previously infected animals and also indicates that sterilising immunity is shorter than the productive life of pigs.ca
dc.format.extent31ca
dc.language.isoengca
dc.publisherWileyca
dc.relation.ispartofTransboundary and Emerging Diseasesca
dc.rightsCopyright © 2021 Wileyca
dc.titleThe immune response does not prevent homologous Porcine epidemic diarrhoea virus reinfection five months after the initial challengeca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/embargoedAccess
dc.date.embargoEnd2022-03-04T01:00:00Z
dc.embargo.terms12 mesosca
dc.relation.projectIDINIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/E-RTA2015-00003- C02-01/ES/Nuevos virus porcinos causantes de diarrea en España/ca
dc.relation.projectIDINIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/E-RTA2015-00003- C02-02/ES/Nuevos virus porcinos causantes de diarrea en España/ca
dc.relation.projectIDMINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2015-17154/ES/ /ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.1111/tbed.14055ca
dc.contributor.groupSanitat Animalca


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