Show simple item record

dc.contributor.authorBosch-Serra, Dolors
dc.contributor.authorRodríguez, Marcela A.
dc.contributor.authorAvilla, Jesús
dc.contributor.authorSarasúa, María José
dc.contributor.authorMiarnau, Xavier
dc.contributor.otherProducció Vegetalca
dc.date.accessioned2021-05-18T09:17:11Z
dc.date.available2021-05-18T09:17:11Z
dc.date.issued2021-04-07
dc.identifier.citationBosch-Serra, Dolors, Marcela A. Rodríguez, Jesús Avilla, María José Sarasúa, and Xavier Miarnau. 2021. "Esterase, Glutathione S-Transferase And NADPH-Cytochrome P450 Reductase Activity Evaluation In Cacopsylla Pyri L. (Hemiptera: Psyllidae) Individual Adults". Insects 12 (4): 329. doi:10.3390/insects12040329.ca
dc.identifier.issn2075-4450ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1262
dc.description.abstractCacopsylla pyri (L.) (Hemiptera: Psyllidae) is a key pest of pear orchards in Spain. The large number of insecticide treatments necessary for control may be an important contributor to the emergence of resistance. Laboratory toxicity and biochemical assays are necessary to validate the existence of insecticide resistance and establish the underlying mechanisms. All the methodologies developed to evaluate enzyme activity in C. pyri to date have incorporated “pools” of adults to detect minimum activity ranges. In this study, we determined the optimal working conditions for evaluation of the activities of esterase, glutathione S-transferase and NADPH-cytochrome P450 reductase in individual insects via colorimetric methods using a microplate reader. The main factors affecting enzymatic analysis activity, such as enzyme source and substrate concentration, filter wavelength, buffer pH, reaction time and additives, were evaluated for optimization. Determining the frequency of resistant individuals within a population could be used as an indicator for the evolution of insecticide resistance over time. Two laboratory strains, one of them selected with cypermethrin, and two field populations were analyzed for this purpose. The data obtained revealed high values and great variation in the activity ranges of esterase (EST) in the insecticide-selected population as well as in the field populations validating the applied methodologyca
dc.format.extent17ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofInsectsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleEsterase, Glutathione S-Transferase and NADPH-Cytochrome P450 Reductase Activity Evaluation in Cacopsylla pyri L. (Hemiptera: Psyllidae) Individual Adultsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-77373-C2-1-R/ES/Avances en el control intregrado de plagas en cultivos hortofrutícolas: mejora del control biológico por conservación en hortícolas y frutales/CIPTOOLSca
dc.subject.udc632ca
dc.identifier.doihttps://doi.org/10.3390/insects12040329ca
dc.contributor.groupProtecció Vegetal Sostenibleca


Files in this item

 

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/