Show simple item record

dc.contributor.authorViñes, Joaquim
dc.contributor.authorCuscó, Anna
dc.contributor.authorNapp, Sebastian
dc.contributor.authorAlvarez, Julio
dc.contributor.authorSaez-Llorente, Jose Luis
dc.contributor.authorRosàs-Rodoreda, Montserrat
dc.contributor.authorFrancino, Olga
dc.contributor.authorMigura-Garcia, Lourdes
dc.contributor.otherProducció Animalca
dc.date.accessioned2021-05-18T11:45:38Z
dc.date.available2021-05-18T11:45:38Z
dc.date.issued2021-03-17
dc.identifier.citationViñes, Joaquim, Anna Cuscó, Sebastian Napp, Julio Alvarez, Jose Luis Saez-Llorente, Montserrat Rosàs-Rodoreda, Olga Francino, and Lourdes Migura-Garcia. 2021. "Transmission Of Similar Mcr-1 Carrying Plasmids Among Different Escherichia Coli Lineages Isolated From Livestock And The Farmer". Antibiotics 10 (3): 313. doi:10.3390/antibiotics10030313.ca
dc.identifier.issn2079-6382ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/1266
dc.description.abstractColistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1-mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the presence and location of mcr-1, other antibiotic resistance genes, and virulence factors. Eighteen colistin resistant isolates (13 from calves, four from pigs, and one from the farmer) contained mcr-1 associated with plasmids (IncX4, IncI2, and IncHI2), except for two that yielded mcr-1 in the chromosome. Similar plasmids were distributed in different E. coli lineages. Transmission of mcr-1 to the farmer most likely occurred by horizontal gene transfer from E. coli of calf origin, since plasmids were highly similar (99% coverage, 99.97% identity). Moreover, 33 virulence factors, including stx2 for Shiga toxin E. coli (STEC) were detected, highlighting the role of livestock as a reservoir of pathotypes with zoonotic potential.ca
dc.format.extent13ca
dc.language.isoengca
dc.publisherMDPIca
dc.relation.ispartofAntibioticsca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleTransmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmerca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095586-B-C22/ES/Optimization of diagnosis and treatment of postweaning diarrheas in pigs: towards a rational use of antibiotics/ca
dc.subject.udc619ca
dc.identifier.doihttps://doi.org/10.3390/antibiotics10030313ca
dc.contributor.groupSanitat Animalca


Files in this item

 
 

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/