dc.contributor.author | Jin, Jing | |
dc.contributor.author | Gan, Kexin | |
dc.contributor.author | Zhao, Lan | |
dc.contributor.author | Jia, Huijuan | |
dc.contributor.author | Zhu, Yifan | |
dc.contributor.author | Li, Xiongwei | |
dc.contributor.author | Yang, Zhaowei | |
dc.contributor.author | Ye, Zhengwen | |
dc.contributor.author | Cao, Ke | |
dc.contributor.author | Wang, Zhiqiang | |
dc.contributor.author | Yu, Mingliang | |
dc.contributor.author | Zhang, Yuyan | |
dc.contributor.author | Ma, Zhisheng | |
dc.contributor.author | Liu, Hangkong | |
dc.contributor.author | Arús, Pere | |
dc.contributor.author | Akkerdaas, Jaap H. | |
dc.contributor.author | Gao, Zongshan | |
dc.contributor.author | van Ree, Ronald | |
dc.contributor.other | Producció Vegetal | ca |
dc.date.accessioned | 2021-05-25T14:50:42Z | |
dc.date.available | 2021-05-25T14:50:42Z | |
dc.date.issued | 2021-05-14 | |
dc.identifier.citation | Jin, Jing, Kexin Gan, Lan Zhao, Huijuan Jia, Yifan Zhu, Xiongwei Li, and Zhaowei Yang et al. 2021. "Peach Allergen Pru P 1 Content Is Generally Low In Fruit But With Large Variation In Different Varieties". Clinical And Translational Allergy 11 (3). doi:10.1002/clt2.12034. | ca |
dc.identifier.issn | 2045-7022 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/1276 | |
dc.description.abstract | Background: Pru p 1 is a major allergen in peach and nectarine, and the different content in varieties may affect the degree of allergic reactions. This study aimed to quantify Pru p 1 levels in representative peach varieties and select hypoallergenic Pru p 1 varieties.
Methods: To obtain monoclonal and polyclonal antibodies, mice and rabbits, respectively, were immunized with recombinant Pru p 1.01 and Pru p 1.02. The Pru p 1 levels in fruits from 83 representative peach varieties was quantified by sandwich enzyme‐linked immunosorbent assay (sELISA). nPru p 1 was obtained through specific monoclonal antibody affinity purification and confirmed by Western blot and mass spectrometry. The variable Pru p 1 content of selected varieties was evaluated by Western blot and the expression level of encoding Pru p 1 genes by quantitative polymerase chain reaction.
Results: A sELISA method with monoclonal and polyclonal antibodies was built for quantifying Pru p 1 levels in peach. Pru p 1 was mainly concentrated in the peel (0.20–73.44 μg/g, fresh weight), being very low in the pulp (0.05–9.62 μg/g) and not detected in wild peach. For the 78 peach and nectarine varieties, Pru p 1 content varied widely from 0.12 to 6.45 μg/g in whole fruit. We verified that natural Pru p 1 is composed of 1.01 and 1.02 isoallergens, and the Pru p 1 expression level and Pru p 1 band intensity in the immunoblots were in agreement with protein quantity determined by ELISA for some tested varieties. In some cases, the reduced levels of Pru p 1 did not coincide with low Pru p 3 in the same variety in whole fruit, while some ancient wild peach and nectarines contained low levels of both allergens, and late‐ripening yellow flesh varieties were usually highly allergenic.
Conclusion: Pru p 1 content is generally low in peach compared to Pru p 3. Several hypoallergenic Pru p 1 and Pru p 3 varieties, “Zi Xue Tao,” “Wu Yue Xian,” and “May Fire,” were identified, which could be useful in trials for peach allergy patients. | ca |
dc.format.extent | 10 | ca |
dc.language.iso | eng | ca |
dc.publisher | Wiley Open Access | ca |
dc.relation.ispartof | Clinical and Translational Allergy | ca |
dc.rights | Copyright © 2021 The Authors. Clinical and Translational Allergy published by John Wiley and Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology | ca |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.title | Peach allergen Pru p 1 content is generally low in fruit but with large variation in different varieties | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 633 | ca |
dc.identifier.doi | https://doi.org/10.1002/clt2.12034 | ca |
dc.contributor.group | Genòmica i Biotecnologia | ca |