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dc.contributor.authorZhou, Ji Shu
dc.contributor.authorLi, Rui Wen
dc.contributor.authorLin, Ya Qiu
dc.contributor.authorWang, Jian Gang
dc.contributor.authorLi, Yang
dc.contributor.authorYang, Yuan Hao
dc.contributor.authorGisbert, Enric
dc.contributor.authorYu, Hai Bo
dc.contributor.authorJi, Hong
dc.contributor.otherProducció Animalca
dc.date.accessioned2018-12-21T10:21:32Z
dc.date.available2018-12-21T10:21:32Z
dc.date.issued2017-08-10
dc.identifier.citationJi Shu Zhou, Rui Wen Li, Ya Qiu Lin, Jian Gang Wang, Yang Li, Yuan Hao Yang, Enric Gisbert, Hai Bo Yu & Hong Ji (2018) LCFA Uptake and FAT/CD36: molecular cloning, tissue expression and mRNA expression responses to dietary oil sources in grass carp (Ctenopharyngodon idellus), Journal of Applied Animal Research, 46:1, 572-582, DOI: 10.1080/09712119.2017.1361828ca
dc.identifier.issn0971-2119ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/129
dc.description.abstractThe fatty acid translocase (FAT/CD36) plays an important role in trans-membrane uptake of long-chain fatty acids (LCFA) and has been identified and described in many organisms, while little is known about this gene in freshwater fish, and especially in the grass carp (Ctenopharyngodon idellus). In this study, the uptake of LCFA in hepatocytes of C. idellus was firstly determined and then FAT/CD36 gene was cloned; then the gene was sequenced and mRNA levels from different tissues and the effect of dietary oils on its expression were determined. Present data showed that trans-membrane absorption of LCFA was related to the type and concentration of fatty acids (FAs) and it could be inhibited by specific membrane uptake inhibitors. The FAT/CD36 isolated from grass carp contained 1422 bp and the open reading frame was predicted to have 1403 bp (GenBank access number: KU361231.1). FAT/CD36 was highly expressed in the brain and its lowest expression was in the gills. FAT/CD36 expression in white muscle was up-regulated when fish were fed with groundnut oil, whereas it was down-regulated in fish fed fish and linseed oils, respectively. These results indicated n-3 PUFA might inhibit the FAT/CD36 mRNA expression and therefore the oxidation of FAs.ca
dc.format.extent12ca
dc.language.isoengca
dc.publisherTaylor & Francisca
dc.relation.ispartofJournal of Applied Animal Researchca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleLCFA Uptake and FAT/CD36: molecular cloning, tissue expression and mRNA expression responses to dietary oil sources in grass carp (Ctenopharyngodon idellus)ca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc63 - Agricultura. Silvicultura. Zootècnia. Caça. Pescaca
dc.identifier.doihttps://doi.org/10.1080/09712119.2017.1361828ca
dc.contributor.groupAqüiculturaca


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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/