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dc.contributor.authorRambla-Alegre, Maria
dc.contributor.authorLeonardo, Sandra
dc.contributor.authorBarguil, Yann
dc.contributor.authorFlores, Cintia
dc.contributor.authorCaixach, Josep
dc.contributor.authorCampbell, Katrina
dc.contributor.authorElliott, Christopher T.
dc.contributor.authorMaillaud, Claude
dc.contributor.authorBoundy, Michael J.
dc.contributor.authorHarwood, D. Tim
dc.contributor.authorCampàs, Mònica
dc.contributor.authorDiogène, Jorge
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-01-04T11:38:39Z
dc.date.available2019-01-04T11:38:39Z
dc.date.issued2017-12-30
dc.identifier.citationRambla-Alegre, Maria, Sandra Leonardo, Yann Barguil, Cintia Flores, Josep Caixach, Katrina Campbell, and Christopher T. Elliott et al. 2018. "Rapid Screening And Multi-Toxin Profile Confirmation Of Tetrodotoxins And Analogues In Human Body Fluids Derived From A Puffer Fish Poisoning Incident In New Caledonia". Food And Chemical Toxicology 112: 188-193. Elsevier BV. doi:10.1016/j.fct.2017.12.039.ca
dc.identifier.issn0278-6915ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/144
dc.description.abstractIn August 2014, a puffer fish poisoning incidence resulting in one fatality was reported in New Caledonia. Although tetrodotoxin (TTX) intoxication was established from the patients' signs and symptoms, the determination of TTX in the patient's urine, serum or plasma is essential to confirm the clinical diagnosis. To provide a simple cost-effective rapid screening tool for clinical analysis, a maleimide-based enzyme-linked immunosorbent assay (mELISA) adapted for the determination of TTX contents in human body fluids was assessed. The mELISA was applied to the analysis of urine samples from two patients and a response for the presence of TTX and/or structurally similar analogues was detected in all samples. The analysis by LC-MS/MS confirmed the presence of TTX but also TTX analogues (4-epiTTX, 4,9-anhydroTTX and 5,6,11-trideoxyTTX) in the urine. A change in the multi-toxin profile in the urine based on time following consumption was observed. LC-MS/MS analysis of serum and plasma samples also revealed the presence of TTX (32.9 ng/mL) and 5,6,11-trideoxyTTX (374.6 ng/mL) in the post-mortem plasma. The results provide for the first time the TTX multi-toxin profile of human samples from a puffer fish intoxication and clearly demonstrate the implication of TTX as the causative agent of the reported intoxication case.ca
dc.format.extent24ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofFood and Chemical Toxicologyca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleRapid screening and multi-toxin profile confirmation of tetrodotoxins and analogues in human body fluids derived from a puffer fish poisoning incident in New Caledoniaca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.terms12 mesosca
dc.relation.projectIDINIA/Programa Estatal de Promoción de Talento y su Empleabilidad en I+D+I/RTA2013-00096-00-00/ES/Promoción de una acuicultura de bivalvos y pesqueria sostenible y segura mediante modelizacióin y caracterización del riesgo de toxinas emergentes/PROMAQUAca
dc.subject.udc574 - Ecologia general i biodiversitatca
dc.identifier.doihttps://doi.org/10.1016/j.fct.2017.12.039ca
dc.contributor.groupAigües Marines i Continentalsca


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/
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