dc.contributor.author | Campàs, Mònica | |
dc.contributor.author | Leonardo, Sandra | |
dc.contributor.author | Oshiro, Naomasa | |
dc.contributor.author | Kuniyoshi, Kyoko | |
dc.contributor.author | Tsumuraya, Takeshi | |
dc.contributor.author | Hirama, Masahiro | |
dc.contributor.author | Diogène, Jorge | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2021-12-13T14:29:00Z | |
dc.date.available | 2022-12-02T23:45:18Z | |
dc.date.issued | 2021-12-02 | |
dc.identifier.citation | Campàs, Mònica, Sandra Leonardo, Naomasa Oshiro, Kyoko Kuniyoshi, Takeshi Tsumuraya, Masahiro Hirama, and Jorge Diogène. 2021. "A Smartphone-Controlled Amperometric Immunosensor For The Detection Of Pacific Ciguatoxins In Fish". Food Chemistry, 131687. doi:10.1016/j.foodchem.2021.131687. | ca |
dc.identifier.issn | 0308-8146 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/1451 | |
dc.description.abstract | Ciguatoxins (CTXs) are marine neurotoxins produced by microalgae of the genera Gambierdiscus and Fukuyoa. CTXs may reach humans through food webs and cause ciguatera fish poisoning (CFP). An immunosensor for the detection of Pacific CTXs in fish was developed using multiwalled carbon nanotube (MWCNT)-modified carbon electrodes and a smartphone-controlled potentiostat. The biosensor attained a limit of detection (LOD) and a limit of quantification (LOQ) of 6 and 27 pg/mL of CTX1B, respectively, which were 0.001 and 0.005 μg/kg in fish flesh. In the analysis of fish samples from Japan and Fiji, excellent correlations were found with sandwich enzyme-linked immunosorbent assays (ELISAs), a cell-based assay (CBA) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Stability of at least 3 months at −20 °C was predicted. In just over 2 h, the biosensor provides reliable, accurate and precise Pacific CTX contents in fish extracts, being suitable for monitoring and research programs. | ca |
dc.format.extent | 26 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | Food Chemistry | ca |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | ca |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | A smartphone-controlled amperometric immunosensor for the detection of Pacific ciguatoxins in fish | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/Dispositivos bioanalíticos para la detección rápida y rentable de gambierdiscus y ciguatoxinas/CIGUASENSING | ca |
dc.relation.projectID | EFSA/ /GP-EFSA-AFSCO-2015-03/EU/Risk Characterization of Ciguatera Food Poisoning in Europe/EUROCIGUA | ca |
dc.relation.projectID | MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA | ca |
dc.subject.udc | 574 | ca |
dc.identifier.doi | https://doi.org/10.1016/j.foodchem.2021.131687 | ca |
dc.contributor.group | Aigües Marines i Continentals | ca |