Electrochemical biosensor for the dual detection of Gambierdiscus australes and Gambierdiscus excentricus in field samples. First report of G. excentricus in the Balearic Islands
Author
Cucchi, Francesca
Toldrà, Anna
Andree, Karl B.
Rey, María
Tsumuraya, Takeshi
O'Sullivan, Ciara K.
Publication date
2021-10-13ISSN
0048-9697
Abstract
Several genera of marine dinoflagellates are known to produce bioactive compounds that affect human health. Among them, Gambierdiscus and Fukuyoa stand out for their ability to produce several toxins, including the potent neurotoxic ciguatoxins (CTXs), which accumulate through the food web. Once fishes contaminated with CTXs are ingested by humans, it can result in an intoxication named ciguatera. Within the two genera, only some species are able to produce toxins, and G. australes and G. excentricus have been highlighted to be the most abundant and toxic. Although the genera Gambierdiscus and Fukuyoa are endemic to tropical areas, their presence in subtropical and temperate regions has been recently recorded. In this work, the combined use of species-specific PCR primers for G. australes and G. excentricus modified with short oligonucleotide tails allowed the development of a multiplex detection system for these two toxin-producing species. Simultaneous detection was achieved using capture probes specific for G. australes and G. excentricus immobilized on maleimide-coated magnetic beads (MBs), separately placed on the working electrodes of a dual electrode array. Additionally, a rapid DNA extraction technique based on a portable bead beater system and MBs was developed, significantly reducing the extraction time (from several hours to 30 min). The developed technique was able to detect as low as 10 cells of both Gambierdiscus species and allowed the first detection of G. excentricus in the Balearic Islands in 8 out of the 9 samples analyzed. Finally, field samples were screened for CTXs with an immunosensor, successfully reporting 13.35 ± 0.5 pg CTX1B equiv. cell−1 in one sample and traces of toxins in 3 out of the 9 samples analyzed. These developments provide rapid and cost-effective strategies for ciguatera risk assessment, with the aim of guaranteeing seafood safety.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
574 - General ecology and biodiversity
637 - Produce of domestic (farmyard) animals and game
Pages
37
Publisher
Elsevier
Is part of
Science of The Total Environment
Citation
Gaiani, Greta, Francesca Cucchi, Anna Toldrà, Karl B. Andree, María Rey, Takeshi Tsumuraya, Ciara K. O'Sullivan, Jorge Diogène, and Mònica Campàs. 2021. "Electrochemical Biosensor For The Dual Detection Of Gambierdiscus Australes And Gambierdiscus Excentricus In Field Samples. First Report Of G. Excentricus In The Balearic Islands". Science Of The Total Environment, 150915. doi:10.1016/j.scitotenv.2021.150915.
Grant agreement number
MINECO-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/Dispositivos bioanalíticos para la detección rápida y rentable de gambierdiscus y ciguatoxinas/CIGUASENSING
EFSA/ /GP-EFSA-AFSCO-2015-03/EU/Risk Characterization of Ciguatera Food Poisoning in Europe/EUROCIGUA
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/PID2019-108781RR-C21/ES/Los riesgos alimentarios asociados con las ciguatoxinas: seguimiento y supervisión de las toxinas marinas y organismos productores de toxinas en los ecosistemas marinos/CIGUARISK
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
Program
Aigües Marines i Continentals
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2510]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/