GreenTray® TIS bioreactor as an effective in vitro culture system for the micropropagation of Prunus spp. rootstocks and analysis of the plant-PGPMs interactions
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Author
Mendoza, Carlos Rolando
Vilaró, Francesca
Publication date
2021-09-23ISSN
0304-4238
Abstract
The use of the GreenTray® TIS bioreactor for the in vitro analysis of the interaction between plantlets and two Plant Growth-Promoting Microorganisms (PGPMs) (Pseudomonas oryzihabitans PGP01 and Cladosporium ramotenellum PGP02) is reported herein. This in vitro culture system improved micropropagation of Prunus Rootpac 20® rootstock, showing greater shoot length and fresh weight compared with culture in semisolid agar-containing medium. Plant responses in co-culture with the PGPMs, their ability to control endophytes growth in the culture media and hormonal changes associated to plant growth were studied in the GreenTray® culture system. Inoculation with P. oryzihabitans PGP01 had no significant effects, whereas C. ramotenellum PGP02 considerably reduced endophytes population after 5 days post inoculation. However, the fungi overgrew the plants roots seriously reducing their growth. These results suggested the effect of the medium pH as a factor to control microbial endophytic growth in the culture medium. A higher P. oryzihabitans PGP01 concentration was inoculated in culture media previously adjusted to pH 5.7 or 7, observing an increased number of roots when plantlets were co-cultured for 15 days with the bacterium in medium adjusted to pH 5.7. This was associated with changes in the levels of IAA in the culture medium, and higher leaf chlorophyll contents than in the non-inoculated cultured plantlets. P. oryzihabitans PGP01 was not able to reduce endophytes growth in either of both media pHs. Altogether, the GreenTray® bioreactor was shown as an efficient system to in vitro micropropagate RP-20 explants as well as to monitor its interaction with PGPMs.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
38
Publisher
Elsevier
Is part of
Scientia Horticulturae
Citation
Cantabella, Daniel, Carlos Rolando Mendoza, Neus Teixidó, Francesca Vilaró, Rosario Torres, and Ramon Dolcet-Sanjuan. 2022. "Greentray® TIS Bioreactor As An Effective In Vitro Culture System For The Micropropagation Of Prunus Spp. Rootstocks And Analysis Of The Plant-Pgpms Interactions". Scientia Horticulturae 291: 110622. doi:10.1016/j.scienta.2021.110622
Grant agreement number
EC/H2020/720719/EU/Agri and food waste valorisation co-ops based on flexible multi-feedstocks biorefinery processing technologies for new high added value applications/AgriMax
Program
Postcollita
Fructicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2685]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/