Assessment of marine benthic diatom communities: insights from a combined morphological–metabarcoding approach in Mediterranean shallow coastal waters
Visualitza/Obre
Autor/a
Pérez-Burillo, Javier
Valoti, Greta
Witkowski, Andrezej
Prado, Patricia
Mann, David G.
Data de publicació
2022-01-18ISSN
0025-326X
Resum
We investigated the advantages and disadvantages of light microscope (LM)-based identifications and DNA metabarcoding, based on a 312-bp rbcL marker, for examining benthic diatom communities from Mediterranean shallow coastal environments. For this, we used biofilm samples collected from different substrata in the Ebro delta bays. We show that 1) Ebro delta bays harbour high-diversity diatom communities [LM identified 249 taxa] and 2) DNA metabarcoding effectively reflects this diversity at genus- but not species level, because of the incompleteness of the DNA reference library. Nevertheless, DNA metabarcoding offers new opportunities for detecting small, delicate and rare diatom species missed by LM and diatoms that lack silica frustules. The primers used, though designed for diatoms, successfully amplified rarely reported members of other stramenopile groups. Combining LM and DNA approaches offers stronger support for ecological studies of benthic microalgal communities in shallow coastal environments than using either approach on its own.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
English
Matèries (CDU)
574 - Ecologia general i biodiversitat
Pàgines
54
Publicat per
Elsevier
Publicat a
Marine Pollution Bulletin
Citació
Pérez-Burillo, Javier, Greta Valoti, Andrzej Witkowski, Patricia Prado, David G. Mann, and Rosa Trobajo. 2022. "Assessment Of Marine Benthic Diatom Communities: Insights From A Combined Morphological–Metabarcoding Approach In Mediterranean Shallow Coastal Waters". Marine Pollution Bulletin 174: 113183. doi:10.1016/j.marpolbul.2021.113183.
Número de l'acord de la subvenció
EC/COST/CA15219/EU/Developing new genetic tools for bioassessment of aquatic ecosystems in Europe/DNAqua-Net
Programa
Aigües Marines i Continentals
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