CRISPR/Cas9 gene editing uncovers the roles of CONSTITUTIVE TRIPLE RESPONSE 1 and REPRESSOR OF SILENCING 1 in melon fruit ripening and epigenetic regulation
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Author
Giordano, Andrea
Santo Domingo, Miguel
Quadrana, Leandro
Pujol, Marta
Martín-Hernández, Ana Montserrat
Publication date
2022-04-08ISSN
0022-0957
Abstract
Melon (Cucumis melo) has emerged as an alternative model to tomato for studying fruit ripening due to the coexistence of climacteric and non-climacteric varieties. Previous characterization of a major quantitative trait locus (QTL), ETHQV8.1, that is able to trigger climacteric ripening in a non-climacteric background resulted in the identification of a negative regulator of ripening CTR1-like (MELO3C024518) and a putative DNA demethylase ROS1 (MELO3C024516) that is the orthologue of DML2, a DNA demethylase that regulates fruit ripening in tomato. To understand the role of these genes in climacteric ripening, in this study we generated homozygous CRISPR knockout mutants of CTR1-like and ROS1 in a climacteric genetic background. The climacteric behavior was altered in both loss-of-function mutants in two growing seasons with an earlier ethylene production profile being observed compared to the climacteric wild type, suggesting a role of both genes in climacteric ripening in melon. Single-cytosine methylome analyses of the ROS1-knockout mutant revealed changes in DNA methylation in the promoter regions of the key ripening genes such as ACS1, ETR1, and ACO1, and in transcription factors associated with ripening including NAC-NOR, RIN, and CNR, suggesting the importance of ROS1-mediated DNA demethylation for triggering fruit ripening in melon.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
633 - Cultius i produccions
Pages
25
Publisher
Oxford University Press
Is part of
Journal of Experimental Botany
Citation
Giordano, Andrea, Miguel Santo Domingo, Leandro Quadrana, Marta Pujol, Ana Montserrat Martín-Hernández, and Jordi Garcia-Mas. 2022. "CRISPR/Cas9 Gene Editing Uncovers The Roles Of CONSTITUTIVE TRIPLE RESPONSE 1 And REPRESSOR OF SILENCING 1 In Melon Fruit Ripening And Epigenetic Regulation". Journal Of Experimental Botany. doi:10.1093/jxb/erac148.
Grant agreement number
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ /
EC/H2020/793090/EU/Implementation of CRISPR/Cas9 technology in melon to edit fruit ripening and CMV resistant genes/MeloCRISP
EC/H2020/948674/EU/GENETIC AND ENVIRONMENTAL BASIS OF NATURAL TRANSPOSITION AND ITS POTENTIAL TO CREATE ADAPTIVE VARIATION/GENTE_Pop
MICIU-FEDER/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-097665-B-C2/ES/APROXIMACIONES DE VARIABILIDAD NATURAL Y EDICIÓN GENÉTICA PARA EL ESTUDIO DE LA CALIDAD DE FRUTO Y LA RESISTENCIA A ENFERMEDADES EN MELÓN/
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2153]
Rights
Copyright © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved.