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dc.contributor.authorHerrera-Uribe, Júber
dc.contributor.authorJiménez-Marín, Ángeles
dc.contributor.authorLacasta, Anna
dc.contributor.authorL. Monteagudo, Paula
dc.contributor.authorPina-Pedrero, Sonia
dc.contributor.authorRodríguez, Fernando
dc.contributor.authorMoreno, Ángela
dc.contributor.authorGarrido, Juan J.
dc.contributor.otherProducció Animalca
dc.date.accessioned2019-02-06T10:17:22Z
dc.date.available2019-02-06T10:17:22Z
dc.date.issued2018-09-12
dc.identifier.citationHerrera-Uribe, Júber, Ángeles Jiménez-Marín, Anna Lacasta, Paula L. Monteagudo, Sonia Pina-Pedrero, Fernando Rodríguez, Ángela Moreno, and Juan J. Garrido. 2018. "Comparative Proteomic Analysis Reveals Different Responses In Porcine Lymph Nodes To Virulent And Attenuated Homologous African Swine Fever Virus Strains". Veterinary Research 49 (1). Springer Nature America, Inc. doi:10.1186/s13567-018-0585-z.ca
dc.identifier.issn0928-4249ca
dc.identifier.urihttp://hdl.handle.net/20.500.12327/180
dc.description.abstractAfrican swine fever (ASF) is a pathology of pigs against which there is no treatment or vaccine. Understanding the equilibrium between innate and adaptive protective responses and immune pathology might contribute to the development of strategies against ASFV. Here we compare, using a proteomic approach, the course of the in vivo infection caused by two homologous strains: the virulent E75 and the attenuated E75CV1. Our results show a progres‑ sive loss of proteins by day 7 post-infection (pi) with E75, refecting tissue destruction. Many signal pathways were afected by both infections but in diferent ways and extensions. Cytoskeletal remodelling and clathrin-endocytosis were afected by both isolates, while a greater number of proteins involved on infammatory and immunological pathways were altered by E75CV1. 14-3-3 mediated signalling, related to immunity and apoptosis, was inhibited by both isolates. The implication of the Rho GTPases by E75CV1 throughout infection is also evident. Early events refected the lack of E75 recognition by the immune system, an evasion strategy acquired by the virulent strains, and signifcant changes at 7 days post-infection (dpi), coinciding with the peak of infection and the time of death. The protein signature at day 31 pi with E75CV1 seems to refect events observed at 1 dpi, including the upregulation of proteosomal subunits and molecules described as autoantigens (vimentin, HSPB1, enolase and lymphocyte cytosolic protein 1), which allow the speculation that auto-antibodies could contribute to chronic ASFV infections. Therefore, the use of proteomics could help understand ASFV pathogenesis and immune protection, opening new avenues for future research.ca
dc.format.extent15ca
dc.language.isoengca
dc.publisherBioMed Centralca
dc.relation.ispartofVeterinary Researchca
dc.rightsAttribution 4.0 Internationalca
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleComparative proteomic analysis reveals different responses in porcine lymph nodes to virulent and attenuated homologous African swine fever virus strainsca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.relation.projectIDMINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22229-C03-01/ES/Caracterización de los mecanismos inmunológicos implicados en protección frente al virus de la peste porcina africana: desarrollo de vacuna frente al VPPA/ca
dc.relation.projectIDMICINN/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-87415-R/ES/Análisis de la interacción patógeno-microbiota-hospedador para entender los mecanismos de colonización y persistencia de Salmonella en el intestino porcino/ca
dc.subject.udc619 - Veterinàriaca
dc.identifier.doihttps://doi.org/10.1186/s13567-018-0585-zca
dc.contributor.groupSanitat Animalca


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