Morphological description of the midgut tract and midgut–hindgut junction in the larvae of the spider crab Maja brachydactyla Balss, 1922 (Malacostraca: Decapoda)

Abstract

The midgut tract of decapods is a digestive organ involved in the synthesis of peritrophic membrane, food transport, absorption of nutrients, and osmoregulation. The midgut tract has been described in detail in adult decapods, but little information is available regarding the morphology and ultrastructure of the midgut tract in larval stages. The present study describes the midgut tract and the midgut–hindgut junction of the larvae of the common spider crab Maja brachydactyla Balss, 1922 using techniques that included dissection, light microscopy, and electron microscopy. The study is mainly focused on the stages of zoea I and megalopa. The results obtained in this study show that the larval midgut tract is a short and simple tube positioned anteriorly, between the stomach and the hindgut tract. During larval development, the maximum length of the midgut tract increases significantly, but no differences were found on either the maximum diameter or the morphological traits of the organ. The midgut tract is active at least ca. 12 h after hatching, as suggested by the presence of the peritrophic membrane in the lumen, the presence of abundant electro-dense vesicles in the cell apex, and the release of the vesicle content on the organ lumen. The midgut–hindgut junction forms an abrupt transition between the midgut tract and the hindgut tract in which epithelial cells with mixed features of midgut and hindgut do not occur.