dc.contributor.author | Puente, Héctor | |
dc.contributor.author | Díaz, Ivan | |
dc.contributor.author | Arguello, Héctor | |
dc.contributor.author | Mencía-Ares, Óscar | |
dc.contributor.author | Gómez-García, Manuel | |
dc.contributor.author | Pérez-Perez, Lucía | |
dc.contributor.author | Vega, Clara | |
dc.contributor.author | Cortey, Martí | |
dc.contributor.author | Martín, Margarita | |
dc.contributor.author | Rubio, Pedro | |
dc.contributor.author | Carvajal, Ana | |
dc.contributor.other | Producció Animal | ca |
dc.date.accessioned | 2022-09-22T14:06:14Z | |
dc.date.available | 2023-08-02T22:45:17Z | |
dc.date.issued | 2022-08-02 | |
dc.identifier.citation | Puente, Héctor, Ivan Díaz, Héctor Arguello, Óscar Mencía‐Ares, Manuel Gómez‐García, Lucía Pérez‐Perez, and Clara Vega et al. 2022. "Characterization And Cross‐Protection Of Experimental Infections With Secov And Two PEDV Variants". Transboundary And Emerging Diseases. doi:10.1111/tbed.14674. | ca |
dc.identifier.issn | 1865-1674 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/1890 | |
dc.description.abstract | The aim of this study was to characterize the infection of weaned pigs with swine enteric coronavirus (SeCoV) -a chimeric virus most likely originated from a recombination event between porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus, or its mutant porcine respiratory coronavirus-, and two PEDV G1b variants, including a recently described recombinant PEDV-SeCoV (rPEDV-SeCoV), as well as to determine the degree of cross-protection achieved against the rPEDV-SeCoV.
For this purpose, forty-eight 4-week-old weaned pigs were randomly allocated into four groups of 12 animals; piglets within each group were primary inoculated with one of the investigated viral strains (B: PEDV; C: SeCoV and D: rPEDV-SeCoV) or mock-inoculated (A), and exposed to rPEDV-SeCOV at day 20 post-infection; thus, group A was primary challenged (-/rPEDV-SeCoV), groups B and C were subjected to a heterologous re-challenge (PEDV/rPEDV-SeCoV and SeCoV/rPEDV-SeCoV, respectively), and group D to a homologous re-challenge (rPEDV-SeCoV/rPEDV-SeCoV), Clinical signs, viral shedding, microscopic lesions and specific humoral and cellular immune responses (IgG, IgA, neutralizing antibodies and IgA and IFN-γ-secreting cells) were monitored.
After primo-infection all three viral strains induced an undistinguishable mild-to-moderate clinical disease with diarrhea as the main sign and villus shortening lesions in the small intestine. In homologous re-challenged pigs, no clinical signs or lesions were observed, and viral shedding was only detected in a single animal. This fact may be explained by the significant high level of rPEDV-SeCoV-specific neutralizing antibodies found in these pigs before the challenge. In contrast, prior exposure to a different PEDV G1b variant or SeCoV only provided partial cross-protection, allowing rPEDV-SeCoV replication and shedding in feces. | ca |
dc.format.extent | 39 | ca |
dc.language.iso | eng | ca |
dc.publisher | Wiley | ca |
dc.relation.ispartof | Transboundary and Emerging Diseases | ca |
dc.rights | Copyright © 2022 Wiley-VCH GmbH. | ca |
dc.title | Characterization and cross-protection of experimental infections with SeCoV and two PEDV variants | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/E-RTA2015-00003-C02-01/ES/Nuevos virus porcinos causantes de diarrea en España/ | ca |
dc.relation.projectID | INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/E-RTA2015-00003-C02-02/ES/Nuevos virus porcinos causantes de diarrea en España/ | ca |
dc.relation.projectID | MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2015-17154/ES// | ca |
dc.subject.udc | 619 | ca |
dc.identifier.doi | https://doi.org/10.1111/tbed.14674 | ca |
dc.contributor.group | Sanitat Animal | ca |