Transcriptome analysis of flathead grey mullet (Mugil cephalus) ovarian development induced by recombinant gonadotropin hormones
Author
Danis, Theodoros
Angelova, Nelina
Tsakogiannis, Alexandros
Giménez, Ignacio
Tsigenopoulos, Costas S.
Manousaki, Tereza
Publication date
2022-11-01ISSN
1664-042X
Abstract
Background: Treatment with recombinant gonadotropin hormones (rGths), follicle-stimulating hormone (rFsh) and luteinizing hormone (rLh), was shown to induce and complete vitellogenesis to finally obtain viable eggs and larvae in the flathead grey mullet (Mugil cephalus), a teleost arrested at early stages of gametogenesis in intensive captivity conditions. This study aimed to investigate the transcriptomic changes that occur in the ovary of females during the rGths-induced vitellogenesis.
Methods: Ovarian samples were collected through biopsies from the same five females at four stages of ovarian development. RNASeq libraries were constructed for all stages studied, sequenced on an Illumina HiSeq4000, and a de novo transcriptome was constructed. Differentially expressed genes (DEGs) were identified between stages and the functional properties of DEGs were characterized by comparison with the gene ontology and Kyoto Encyclopedia. An enrichment analysis of molecular pathways was performed.
Results: The de novo transcriptome comprised 287,089 transcripts after filtering. As vitellogenesis progressed, more genes were significantly upregulated than downregulated. The rFsh application induced ovarian development from previtellogenesis to early-to-mid-vitellogenesis with associated pathways enriched from upregulated DEGs related to ovarian steroidogenesis and reproductive development, cholesterol metabolism, ovarian growth and differentiation, lipid accumulation, and cell-to-cell adhesion pathways. The application of rFsh and rLh at early-to-mid-vitellogenesis induced the growth of oocytes to late-vitellogenesis and, with it, the enrichment of pathways from upregulated DEGs related to the production of energy, such as the lysosomes activity. The application of rLh at late-vitellogenesis induced the completion of vitellogenesis with the enrichment of pathways linked with the switch from vitellogenesis to oocyte maturation.
Conclusion: The DEGs and enriched molecular pathways described during the induced vitellogenesis of flathead grey mullet with rGths were typical of natural oogenesis reported for other fish species. Present results add new knowledge to the rGths action to further raise the possibility of using rGths in species that present similar reproductive disorders in aquaculture, the aquarium industry as well as the conservation of endangered species.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
639 - Hunting. Fishing. Fish breeding
Pages
17
Publisher
Frontiers Media
Is part of
Frontiers in Physiology
Citation
Ramos-Júdez, Sandra, Theodoros Danis, Nelina Angelova, Alexandros Tsakogiannis, Ignacio Giménez, Costas S. Tsigenopoulos, Neil Duncan, and Tereza Manousaki. 2022. "Transcriptome Analysis Of Flathead Grey Mullet (Mugil Cephalus) Ovarian Development Induced By Recombinant Gonadotropin Hormones". Frontiers In Physiology 13. doi:10.3389/fphys.2022.1033445.
Grant agreement number
EC/H2020/652831/EU/AQUAculture infrastructures for EXCELlence in European fish research towards 2020/AQUAEXCEL2020
EC/FP7/264089/EU/Supporting research potential for MARine BIodiversity and GENomics in the Eastern Mediterranean/MARBIGEN
MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-094710-R-I00/ES/CERRANDO CIRCULOS BIOLOGICOS Y ECONOMICOS PARA EL PARDETE (MUGIL CEPHALUS)/
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2811]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/