A proof‑of‑concept study to investigate the efcacy of heat‑inactivated autovaccines in Mycobacterium caprae experimentally challenged goats
Arrieta-Villegas, Claudia Cecilia
Sevilla, Iker A.
Garrido, Joseba M.
This study aimed to assess the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats experimentally challenged with the same strain of M. caprae. Twenty-one goats were divided into three groups of seven: vaccinated with heat-inactivated Mycobacterium bovis (HIMB), with HIMC and unvaccinated. At 7 weeks post-vaccination all animals were endobronchially challenged with M. caprae. Blood samples were collected for immunological assays and clinical signs were recorded throughout the experiment. All goats were euthanized at 9 weeks post-challenge. Gross pathological examination, analysis of lung pathology using computed tomography, and bacterial load quantification in pulmonary lymph nodes (LN) by qPCR were carried out. Only HIMC vaccinated goats showed a significant reduction of lung lesions volume and mycobacterial DNA load in LN compared to unvaccinated controls. Both vaccinated groups showed also a significant reduction of the other pathological parameters, an improved clinical outcome and a higher proportion of IFN-γ-producing central memory T cells after vaccination. The results indicated that homologous vaccination of goats with HIMC induced enhanced protection against M. caprae challenge by reducing lung pathology and bacterial load compared to the heterologous vaccine (HIMB). Further large-scale trials are necessary to assess the efficacy of autovaccines under field conditions.
619 - Veterinària
Is part of
Melgarejo, Cristian, Carles Planas, Alex Cobos, Claudia Arrieta-Villegas, Iker A. Sevilla, Javier Bezos, Xavier Moll, Yvonne Espada, Joseba M. Garrido, Mariano Domingo, Enric Vidal, and Bernat Pérez de Val. 2022. "A Proof-Of-Concept Study To Investigate The Efficacy Of Heat-Inactivated Autovaccines In Mycobacterium Caprae Experimentally Challenged Goats". Scientific Reports 12 (1). doi:10.1038/s41598-022-26683-0.
Grant agreement number
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-105155RB-C32/ES/ /
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- ARTICLES CIENTÍFICS 
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