The FlagT4G Vaccine Confers a Strong and Regulated Immunity and Early Virological Protection against Classical Swine Fever
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Author
Wang, Miaomiao
Díaz, Ivan
Alberch, Mònica
Pérez-Simó, Marta
Gladue, Douglas P.
Borca, Manuel V.
Publication date
2022-09-02ISSN
1999-4915
Abstract
Control of classical swine fever virus (CSFV) in endemic countries relies on vaccination, mostly using vaccines that do not allow for differentiation of vaccinated from infected animals (DIVA). FlagT4G vaccine is a novel candidate that confers robust immunity and shows DIVA capabilities. The present study assessed the immune response elicited by FlagT4G and its capacity to protect pigs for a short time after vaccination. Five days after a single dose of FlagT4G vaccine, animals were challenged with a highly virulent CSFV strain. A strong, but regulated, interferon-α response was found after vaccination. Vaccinated animals showed clinical and virological protection against the challenge, in the absence of antibody response at 5 days post-vaccination. Upon challenge, a rapid rise in the titers of CSFV neutralizing antibodies and an increase in the IFN-γ producing cells were noticed in all vaccinated-challenged pigs. Meanwhile, unvaccinated pigs showed severe clinical signs and high viral replication, being euthanized before the end of the trial. These animals were unable to generate neutralizing antibodies and IFN-γ responses after the CSFV challenge. The results from the present study assert the fast and efficient protection by FlagT4G, a highly promising tool for CSFV control worldwide.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
16
Publisher
MDPI
Is part of
Viruses
Citation
Bohórquez, José Alejandro, Miaomiao Wang, Ivan Díaz, Mònica Alberch, Marta Pérez-Simó, Rosa Rosell, Douglas P. Gladue, Manuel V. Borca, and Llilianne Ganges. 2022. "The Flagt4g Vaccine Confers A Strong And Regulated Immunity And Early Virological Protection Against Classical Swine Fever". Viruses 14 (9): 1954. doi:10.3390/v14091954.
Grant agreement number
MINECO/Programa estatal de I+D+I orientada a los retos de la sociedad/RTI2018-100887-B-100/ES/Descifrando nuevos factores virales y del hospedador involucrados en el desarrollo de la peste porcina clásica: implicaciones para el control de la enfermedad/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/