Rhizosphere Acidification as the Main Trait Characterizing the Differential In Vitro Tolerance to Iron Chlorosis in Interspecific Pyrus Hybrids
Author
Mora-Córdova, Claudia Paola
Tolrà, Roser P.
Padilla, Rosa
Poschenrieder, Charlotte
Simard, Marie-Helene
Claveria, Elisabet
Publication date
2022-06-18ISSN
2311-7524
Abstract
Physiological responses of different interspecific Pyrus hybrids and an open pollinated Pyrus
communis ‘Williams’ (Pcw) grown under in vitro culture conditions simulating lime induced chlorosis
were studied. The hybrids were derived from crosses between the ‘Pyriam’ pear rootstock and four
Pyrus species of the Mediterranean region, namely P. amygdaliformis Vill. (Pa), P. amygdaliformis persica
Bornme. (Pap), P. communis cordata (Desv.) Hook. (Pcc), and P. elaeagrifolia Pall (Pe), all known for their
higher field tolerance to iron-chlorosis than P. communis. Twenty hybrids and one open pollinated
Pcw were micropropagated, and plantlets were in vitro characterized for their physiological responses
to iron-deficiency conditions. Rooted plantlets were transferred to a culture medium with 2 µM Fe3+
DTPA and 10 or 20 mM NaHCO3. These physiological responses were scored at 1, 3, 7, and 28 days
from the start of the in vitro assay. Leaf total chlorophyll content, the capacity of roots to acidify the
medium, reduced iron, and exudates of phenolic acids and organic acids were analyzed in each media
and time sample. Leaf chlorophyll levels for the clones derived from Pcc were the highest, especially
under the highest bicarbonate concentration, followed by those derived from Pap and Pa. The higher
chlorophyll content of Pcc clones were related with their higher capacity to acidify the media but not
with their iron reduction capacity at the root level. On the other hand, hybrid clones derived from
Pe showed a higher Fe3+ reduction ability than clones from all the other species during the whole
assay but only when the bicarbonate concentration was lower. The exudation of phenolic acids by
the roots was higher in Pcw than in the other species, and this response might explain why the total
chlorophyll levels in Pcw clones are similar to those of Pe and Pa ones. These results with Pyrus spp.
bring more evidence in support of the idea that iron reduction capacity at the root level is not directly
related with a higher tolerance to iron deficiency caused by the high pH of calcareous soils. Instead,
the ability to acidify the rhizosphere is the trait of choice for the selection of the pear hybrid clones
better adapted to lime induced chlorosis. In addition, the in vitro assay to select the Pyrus clones for
tolerance to iron chlorosis could be shortened to one week of culture in 10 mM NaHCO3, measuring
the leaf chlorophyll level, acidification of the culture medium, and exudation of phenolic acids as the
physiological responses to predict tolerance to lime-induced chlorosis
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
632 - Plant damage, injuries. Plant diseases. Pests, organisms injurious to plants. Plant protection
Pages
15
Publisher
MDPI
Is part of
Horticulturae
Citation
Mora-Córdova, Claudia Paola, Roser Tolrà, Rosa Padilla, Charlotte Poschenrieder, Marie-Helene Simard, Luis Asín, Pere Vilardell, Joan Bonany, Elisabet Claveria, and Ramon Dolcet-Sanjuan. 2022. "Rhizosphere Acidification As The Main Trait Characterizing The Differential In Vitro Tolerance To Iron Chlorosis In Interspecific Pyrus Hybrids". Horticulturae 8 (6): 551. doi:10.3390/horticulturae8060551
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/ES/BFU2016-75176-R/MECANISMOS DE ADAPTACION LOCAL DE PLANTAS MODELO AL AMBIENTE IONICO RIZOSFERICO CON ESPECIAL ENFASIS EN LA SALINIDAD DE LOS SUELOS CALCAREOS MEDITERRANEOS/
INIA-FEDER/Programa Nacional de Proyectos de Investigación Fundamental/RTA2012-00049-00-00/ES/Obtención, selección y caracterización fisiológica de nuevos patrones híbridos, tolerantes a clorosis férrica y de vigor reducido, para plantaciones intensivas de peral/
Program
Fructicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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