Non-volatile signals and redox mechanisms are required for the responses of Arabidopsis roots to Pseudomonas oryzihabitans
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Publication date
2022-08-24ISSN
0022-0957
Abstract
Soil bacteria promote plant growth and protect against environmental stresses, but the mechanisms involved remain poorly characterized, particularly when there is no direct contact between the roots and bacteria. Here, we explored the effects of Pseudomonas oryzihabitans PGP01 on the root system architecture (RSA) in Arabidopsis thaliana seedlings. Significant increases in lateral root (LR) density were observed when seedlings were grown in the presence of P. oryzihabitans, as well as an increased abundance of transcripts associated with altered nutrient transport and phytohormone responses. However, no bacterial transcripts were detected on the root samples by RNAseq analysis, demonstrating that the bacteria do not colonize the roots. Separating the agar containing bacteria from the seedlings prevented the bacteria-induced changes in RSA. Bacteria-induced changes in RSA were absent from mutants defective in ethylene response factor (ERF109), glutathione synthesis (pad2-1, cad2-1, and rax1-1) and in strigolactone synthesis (max3-9 and max4-1) or signalling (max2-3). However, the P. oryzihabitans-induced changes in RSA were similar in the low ascorbate mutants (vtc2-1and vtc2-2) to the wild-type controls. Taken together, these results demonstrate the importance of non-volatile signals and redox mechanisms in the root architecture regulation that occurs following long-distance perception of P. oryzihabitans.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
29
Publisher
Oxford University Press
Is part of
Journal of Experimental Botany
Citation
Cantabella, Daniel, Barbara Karpinska, Neus Teixidó, Ramon Dolcet-Sanjuan, and Christine H Foyer. 2022. "Non-Volatile Signals And Redox Mechanisms Are Required For The Responses Of Arabidopsis Roots To Pseudomonas Oryzihabitans". Journal Of Experimental Botany 73 (19): 6971-6982. doi:10.1093/jxb/erac346
Grant agreement number
EC/H2020/720719/EU/Agri and food waste valorisation co-ops based on flexible multi-feedstocks biorefinery processing technologies for new high added value applications/AgriMax
Program
Fructicultura
Postcollita
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
Rights
Copyright © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology