Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate
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Borrego, Carles M.
Pailler García, Lola
Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in waterfowl staging and breeding areas is key to understanding their transmission dynamics and optimizing management strategies. Here, we used artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate (day/night cycles of photosynthetic active radiation and temperature, low water velocity, and similar microbiome to lowland rivers and stagnant water bodies) and then manipulated temperature and sediment presence (i.e., 10–13 °C vs. 16–18 °C, and presence vs. absence of sediments). An H1N1 low pathogenic AIV (LPAIV) strain was spiked in the streams, and water and sediment samples were collected at different time points until 14 days post-spike to quantify viral RNA and detect infectious particles. Viral RNA was detected until the end of the experiment in both water and sediment samples. In water samples, we observed a significant combined effect of temperature and sediments in viral decay, with higher viral genome loads in colder streams without sediments. In sediment samples, we didn't observe any significant effect of temperature. In contrast to prior laboratory-controlled studies that detect longer persistence times, infectious H1N1 LPAIV was isolated in water samples till 2 days post-spike, and none beyond. Infectious H1N1 LPAIV wasn't isolated from any sediment sample. Our results suggest that slow flowing freshwater surface waters may provide conditions facilitating bird-to-bird transmission for a short period when water temperature are between 10 and 18 °C, though persistence for extended periods (e.g., weeks or months) may be less likely. We hypothesize that experiments simulating real environments, like the one described here, provide a more realistic approach for assessing environmental persistence of AIVs.
619 - Veterinary science
Is part of
Science of the Total Environment
Perlas, Albert, Kateri Bertran, Francesc Xavier Abad, Carles M. Borrego, Miquel Nofrarías, Rosa Valle, Lola Pailler-García, Antonio Ramis, Martí Cortey, Vicenç Acuña and Natàlia Majó. 2023. "Persistence Of Low Pathogenic Avian Influenza Virus In Artificial Streams Mimicking Natural Conditions Of Waterfowl Habitats In The Mediterranean Climate". Science Of The Total Environment 863: 160902. doi:10.1016/j.scitotenv.2022.160902.
Grant agreement number
INIA/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTA2015-00088-C03-03/ES/Influenza aviar: Estudio de la dinámica de la infección en aves autóctonas y de persistencia del virus en condiciones ambientales/
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-114060RR-C33/ES/INVESTIGANDO LOS MECANISMOS MOLECULARES DE LA EVOLUCION DE LA INFECCION CON VIRUS DE INFLUENZA AVIAR EN LAS AVES MEDIANTE UNA APROXIMACION MULTI-OMICA/INFLUOMA
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