Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq
Author
Valdés-Hernández, Jesús
Passols, Magí
Sebastià, Cristina
Criado-Mesas, Lourdes
Esteve-Codina, Anna
Castelló, Anna
Sánchez, Armand
Folch, Josep M.
Publication date
2023-01-11ISSN
2045-2322
Abstract
Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis and determining meat quality in pigs. However, FAs are transformed by the action of several genes, such as those encoding desaturases and elongases of FAs in lipogenic tissues. The aim of the current work was to identify candidate genes, biological processes, and pathways involved in the modulation of intramuscular FA profile from longissimus dorsi muscle. FA profile by gas chromatography of methyl esters and gene expression by RNA-Seq were determined in 129 Iberian × Duroc backcrossed pigs. An association analysis between the muscle transcriptome and its FA profile was performed, followed by a concordance and functional analysis. Overall, a list of well-known (e.g., PLIN1, LEP, ELOVL6, SC5D, NCOA2, ACSL1, MDH1, LPL, LGALS12, TFRC, GOT1, and FBP1) and novel (e.g., TRARG1, TANK, ENSSSCG00000011196, and ENSSSCG00000038429) candidate genes was identified, either in association with specific or several FA traits. Likewise, several of these genes belong to biological processes and pathways linked to energy, lipid, and carbohydrate metabolism, which seem determinants in the modulation of FA compositions. This study can contribute to elucidate the complex relationship between gene expression and FA profile in pig muscle.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
663/664 - Food and nutrition. Enology. Oils. Fat
Pages
12
Publisher
Nature Research
Is part of
Scientific reports
Citation
Valdés-Hernández, Jesús, Yuliaxis Ramayo-Caldas, Magí Passols, Cristina Sebastià, Lourdes Criado-Mesas, Daniel Crespo-Piazuelo, Anna Esteve-Codina, Anna Castelló, Armand Sánchez, and Josep M. Folch. 2023. "Global Analysis Of The Association Between Pig Muscle Fatty Acid Composition And Gene Expression Using RNA-Seq". Scientific Reports 13 (1). doi:10.1038/s41598-022-27016-x.
Grant agreement number
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2017-82641-R/ES/GENOMICA FUNCIONAL, BIOLOGIA DE SISTEMAS Y MICROBIOMICA APLICADAS A LA IDENTIFICACION DE REGULADORES GENETICOS DEL CRECIMIENTO, ENGRASAMIENTO Y CALIDAD DE LA CARNE EN PORCINO/
MICIU-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/
ISCIII-FEDER/ /PT17-0009-0019/ES/ /
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ /
MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112677RB-C22/ES/FISIOLOGIA MOLECULAR DEL METABOLISMO ENERGETICO EN PORCINO: BASES PARA LA SELECCION DE LA EFICIENCIA PRODUCTIVA Y CALIDAD DE CARNE/
Program
Genètica i Millora Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2549]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/