Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
Author
Fernández-Veiga, Leire
Fuertes, Miguel
Geijo, María V.
Michelet, Lorraine
Boschiroli, María Laura
Gómez-Buendía, Alberto
Bezos, Javier
Jones, Gareth J.
Vordermeier, Martin
Juste, Ramón A.
Garrido, Joseba M.
Sevilla, Iker A.
Publication date
2023-02-20ISSN
2045-2322
Abstract
The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
12
Publisher
Nature Research
Is part of
Scientific Reports
Citation
Fernández-Veiga, Leire, Miguel Fuertes, María V. Geijo, Bernat Pérez de Val, Enric Vidal, Lorraine Michelet, María Laura Boschiroli, Alberto Gómez-Buendía, Javier Bezos, Gareth J. Jones, Martin Vordermeier, Ramón A. Juste, Joseba M. Garrido and Iker A. Sevilla. 2023. "Differences In Skin Test Reactions To Official And Defined Antigens In Guinea Pigs Exposed To Non-Tuberculous And Tuberculous Bacteria". Scientific Reports 13 (1). doi:10.1038/s41598-023-30147-4.
Grant agreement number
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-105155RB-C33/ES/DEVELOPMENT OF INACTIVATED VACCINE PROTOTYPES FOR THE CONTROL OF TB IN DOMESTIC ANIMALS AND ASSESSMENT OF THE INTEREFERENCE CAUSED BY NON TUBERCULOUS ORGANISMS ON ITS DIAGNOSI/
EC/INTERREG-POCTEFA/EFA357-19/EU/Red de investigación y desarrollo de herramientas innovadoras para el control de la tuberculosis animal/INNOTUB
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2649]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/