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Mutations in CmVPS41 controlling resistance to cucumber mosaic virus display specific subcellular localization
dc.contributor.author | Real, Núria | |
dc.contributor.author | Villar, Irene | |
dc.contributor.author | Serrano, Irene | |
dc.contributor.author | Guiu-Aragonés, Cèlia | |
dc.contributor.author | Martin-Hernandez, Ana Montserrat | |
dc.contributor.other | Producció Vegetal | ca |
dc.date.accessioned | 2023-02-27T12:06:27Z | |
dc.date.available | 2023-12-17T23:45:22Z | |
dc.date.issued | 2022-12-17 | |
dc.identifier.citation | Real, Núria, Irene Villar, Irene Serrano, Cèlia Guiu-Aragonés, and Ana Montserrat Martín-Hernández. 2022. "Mutations In Cmvps41 Controlling Resistance To Cucumber Mosaic Virus Display Specific Subcellular Localization". Plant Physiology. doi:10.1093/plphys/kiac583. | ca |
dc.identifier.issn | 0032-0889 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12327/2128 | |
dc.description.abstract | Resistance to cucumber mosaic virus (CMV) in melon (Cucumis melo L.) has been described in several exotic accessions and is controlled by a recessive resistance gene, cmv1, that encodes a vacuolar protein sorting 41 (CmVPS41). cmv1 prevents systemic infection by restricting the virus to the bundle sheath cells, preventing viral phloem entry. CmVPS41 from different resistant accessions carries two causal mutations, either a G85E change, found in Pat-81 and Freeman’s cucumber, or L348R, found in PI161375, cultivar Songwhan Charmi (SC). Here, we analyzed the subcellular localization of CmVPS41 in Nicotiana benthamiana and found differential structures in resistant and susceptible accessions. Susceptible accessions showed nuclear and membrane spots and many transvacuolar strands, whereas the resistant accessions showed many intravacuolar invaginations. These specific structures colocalized with late endosomes. Artificial CmVPS41 carrying individual mutations causing resistance in the genetic background of CmVPS41 from the susceptible variety Piel de Sapo (PS) revealed that the structure most correlated with resistance was the absence oftransvacuolar strands. Coexpression of CmVPS41 with viral movement proteins, the determinant of virulence, did not change these localizations; however, infiltration of CmVPS41 from either SC or PS accessions in CMV-infected N. benthamiana leaves showed a localization pattern closerto each other, with upto 30% cellsshowingsomemembranespots inthe CmVPS41SC and fewertransvacuolarstrands (reduced from a mean of 4 to 1–2) with CmVPS41PS. Our results suggest that the distribution of CmVPS41PS in late endosomes includes transvacuolar strands that facilitate CMV infection and that CmVPS41 re-localizes during viral infection. | ca |
dc.format.extent | 32 | ca |
dc.language.iso | eng | ca |
dc.publisher | Oxford University Press | ca |
dc.relation.ispartof | Plant Physiology | ca |
dc.rights | Copyright © 2022, Oxford University Press | ca |
dc.title | Mutations in CmVPS41 controlling resistance to cucumber mosaic virus display specific subcellular localization | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/acceptedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.relation.projectID | MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2015-64625-C2-1-R/ES/DISECCION GENETICA DE DOS CARACTERES DE INTERES AGRONOMICO EN MELON: RESISTENCIA A CUCUMBER MOSAIC VIRUS Y MADURACION CLIMATERICA DE FRUTO/RESMELORIP | ca |
dc.relation.projectID | MICIU/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-097665-B-C2/ES/APROXIMACIONES DE VARIABILIDAD NATURAL Y EDICIÓN GENÉTICA PARA EL ESTUDIO DE LA CALIDAD DE FRUTO Y LA RESISTENCIA A ENFERMEDADES EN MELÓN/ | ca |
dc.relation.projectID | EC/H2020/665919/EU/Opening Sphere UAB-CEI to PostDoctoral Fellows/P-SPHERE MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ / | ca |
dc.relation.projectID | MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/CEX2019-000902-S/ES/ / | ca |
dc.relation.projectID | MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/SEV-2015-0533/ES/ / | ca |
dc.relation.projectID | FEDER/ / /EU/ / | ca |
dc.subject.udc | 633 | ca |
dc.identifier.doi | https://doi.org/10.1093/plphys/kiac583 | ca |
dc.contributor.group | Genòmica i Biotecnologia | ca |
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