Identification of microRNAs in PCV2 subclinically infected pigs by high throughput sequencing
View/Open
Author
Núñez-Hernández, Fernando
Pérez, Lester J.
Muñoz, Marta
Vera, Gonzalo
Tomás, Anna
Egea, Raquel
Córdoba, Sarai
Sánchez, Armand
Publication date
2015-03-03ISSN
0928-4249
Abstract
Porcine circovirus type 2 (PCV2) is the essential etiological infectious agent of PCV2-systemic disease and has been associated with other swine diseases, all of them collectively known as porcine circovirus diseases. MicroRNAs (miRNAs) are a new class of small non-coding RNAs that regulate gene expression post-transcriptionally. miRNAs play an increasing role in many biological processes. The study of miRNA-mediated host-pathogen interactions has emerged in the last decade due to the important role that miRNAs play in antiviral defense. The objective of this study was to identify the miRNA expression pattern in PCV2 subclinically infected and non-infected pigs. For this purpose an experimental PCV2 infection was carried out and small-RNA libraries were constructed from tonsil and mediastinal lymph node (MLN) of infected and non-infected pigs. High throughput sequencing determined differences in miRNA expression in MLN between infected and non-infected while, in tonsil, a very conserved pattern
was observed. In MLN, miRNA 126-3p, miRNA 126-5p, let-7d-3p, mir-129a and mir-let-7b-3p were up-regulated whereas mir-193a-5p, mir-574-5p and mir-34a down-regulated. Prediction of functional analysis showed that these miRNAs can be involved in pathways related to immune system and in processes related to the pathogenesis of PCV2, although functional assays are needed to support these predictions. This is the first study on miRNA gene expression in pigs infected with PCV2 using a high throughput sequencing approach in which several host miRNAs were differentially expressed in response to PCV2 infection.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
7
Publisher
BMC
Is part of
Veterinary Research
Citation
Núñez-Hernández, Fernando, Lester J Pérez, Marta Muñoz, Gonzalo Vera, Anna Tomás, Raquel Egea, Sarai Córdoba, Joaquim Segalés, Armand Sánchez, and José I Núñez. 2015. "Identification Of Micrornas In PCV2 Subclinically Infected Pigs By High Throughput Sequencing". Veterinary Research 46 (1): 18. doi:10.1186/s13567-014-0141-4.
Grant agreement number
MEC/ /AGL2007-66371-C02-02/ES/IDENTIFICACION Y CARACTERIZACION DE MICRORNAS VIRICOS QUE AFECTAN AL PORCINO/
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2010-22358-C02-02/ES/MICRORNAS EN INFECCIONES VIRICAS DEL CERDO: ANALISIS FUNCIONAL E IMPLICACIONES EN PATOGENIA VIRAL/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
The following license files are associated with this item:
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/