Bifurcaria bifurcata extract exerts antioxidant effects on human Caco-2 cells
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Autor/a
Martínez, María-Aránzazu
Aedo, Hugo
Lopez-Torres, Bernardo
Maximiliano, Jorge-Enrique
Martínez-Larrañaga, María-Rosa
Anadón, Arturo
Martínez, Marta
Peteiro, Cesar
Cueto, Mercedes
Ares, Irma
Fecha de publicación
2023-05-13ISSN
0013-9351
Resumen
The present research study investigated the potential protective effect of Bifurcaria bifurcata extract on cell viability and antioxidant defences of cultured human Caco-2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (tert-BOOH). Aqueous extracts were firstly characterized in terms of total phenolic contents. Concentrations of reduced glutathione (GSH) and malondialdehyde (MDA), generation of reactive oxygen species (ROS), nitric oxide (NO) production, antioxidant enzymes activities [NADPH quinone dehydrogenase 1 (NQO1) and glutathione S-transferase (GST)], caspase 3/7 activity and gene expression linked to apoptosis, proinflammation and oxidative stress signaling pathways were used as markers of cellular oxidative status. B. bifurcata extract prevented the cytotoxicity, the decrease of GSH, the increase of MDA levels and the ROS generation induced by tert-BOOH. B. bifurcata extract prevented the significant decrease of NQO1 and GST activities, and the significant increase of caspase 3/7 activity induced by tert-BOOH. B. bifurcata extract also caused an over-expression of GSTM2, Nrf2 and AKT1 transcriptors, as well as reduced ERK1, JNK1, Bax, BNIP3, NFκB1, IL-6 and HO-1 gene expressions induced by tert-BOOH suggesting an increase in cellular resistance against oxidative stress. The results of the biomarkers analyzed show that treatment of Caco-2 cells with B. bifurcata extract enhance antioxidant defences, which imply an improved cell response to an oxidative challenge. B. bifurcata extract possesses strong antioxidant properties and may be a potential effective alternative to oxidant agents in the functional food industry.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
English
Materias (CDU)
663/664 - Alimentos y nutrición. Enología. Aceites. Grasas
Páginas
54
Publicado por
Elsevier
Publicado en
Environmental Research
Citación
Martínez, María-Aránzazu, Hugo Aedo, Bernardo Lopez-Torres, Jorge-Enrique Maximiliano, María-Rosa Martínez-Larrañaga, Arturo Anadón, and Marta Martínez et al. 2023. "Bifurcaria Bifurcata Extract Exerts Antioxidant Effects On Human Caco-2 Cells". Environmental Research 231: 116141. doi:10.1016/j.envres.2023.116141.
Número del acuerdo de la subvención
MICINN/Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-115979RR-C33/ES/EVALUACION DE LA TOXICIDAD IN VITRO Y/O FUNCIONALIDAD DE COMPUESTOS ACTIVOS CARACTERIZADOS DE ALGAS PARDAS Y MICROBIOTA MARINA/
Program
Funcionalitat i Seguretat Alimentària
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