Antibiofilm surfaces based on the immobilization of a novel recombinant antimicrobial multidomain protein using self-assembled monolayers
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Author
Kyvik, Adriana R.
Mayolo-Deloisa, Karla
Rodriguez Rodriguez, Xavier
Martinez-Miguel, Marc
Martos, Marta
Köber, Mariana
Ventosa, Nora
Veciana, Jaume
Guasch, Judith
Arís, Anna
Ratera, Imma
Publication date
2023-04-13ISSN
2633-5409
Abstract
The constant increase of microorganisms resistant to antibiotics has been classified as a global health emergency, which is especially challenging when biofilms are formed. Herein, novel biofunctionalized gold surfaces with the antimicrobial multidomain recombinant protein JAMF1, both in the soluble form and nanostructured as nanoparticles, were developed. The interaction between His-tag termination of the protein and a nitriloacetic acid–Ni complex formed on mixed self-assembled monolayers (mixed SAMs) was exploited. The obtained antibiofilm surfaces based on the immobilization of the novel JAMF1 protein using self-assembled monolayers were characterized using a multi-technique approach including: cyclic voltammetry, X-ray photoelectron spectroscopy, atomic force microscopy and fluorescence, proving that the modification and immobilization of JAMF1 were successfully done. The antibiofilm activity against Escherichia coli and carbapenem-resistant Klebsiella pneumoniae showed that the immobilized antimicrobial proteins were able to reduce biofilm formation of both microorganisms. This strategy opens up new possibilities for controlled biomolecule immobilization for fundamental biological studies and biotechnological applications, at the interface of materials science and molecular biology.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
636 - Animal husbandry and breeding in general. Livestock rearing. Breeding of domestic animals
Pages
11
Publisher
Royal Society of Chemistry
Is part of
Materials Advances
Citation
Kyvik, Adriana R., Ramon Roca-Pinilla, Karla Mayolo-Deloisa, Xavier Rodriguez Rodriguez, Marc Martinez-Miguel, Marta Martos, and Mariana Köber et al. 2023. "Antibiofilm Surfaces Based On The Immobilization Of A Novel Recombinant Antimicrobial Multidomain Protein Using Self-Assembled Monolayers". Materials Advances 4 (10): 2354-2364. doi:10.1039/d2ma00978a.
Grant agreement number
MICINN/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-115296RA-I00/ES/MIMETIZANDO EL SISTEMA INMUNITARIO CONTRA EL CANCER/
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2019-105622RB-I00/ES/PROCESAMIENTO DE MOLECULAS PARA CREAR MATERIALES HIBRIDOS ESTRUCTURADOS JERARQUICAMENTE PARA APLICACIONES BIOMEDICAS/
EC/H2020/101092269/EU/ANTIMICROBIAL NANOSTRUCTURED BIOMATERIALS FOR COMPLEX WOUND HEALING/NABIHEAL
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC-2017-22614/ES/ /
Program
Producció de Remugants
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2845]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc/4.0/