Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs
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Author
Muñoz-González, Sara
Perez-Simó, Marta
Muñoz, Marta
Bohorquez, José Alejandro
Summerfield, Artur
Domingo, Mariano
Ruggli, Nicolas
Publication date
2015-07-09ISSN
0928-4249
Abstract
Classical swine fever (CSF) causes major losses in pig farming, with various degrees of disease severity. Efficient live attenuated vaccines against classical swine fever virus (CSFV) are used routinely in endemic countries. However, despite intensive vaccination programs in these areas for more than 20 years, CSF has not been eradicated. Molecular epidemiology studies in these regions suggests that the virus circulating in the field has evolved under the positive selection pressure exerted by the immune response to the vaccine, leading to new attenuated viral variants. Recent work by our group demonstrated that a high proportion of persistently infected piglets can be generated by early postnatal infection with low and moderately virulent CSFV strains. Here, we studied the immune response to a hog cholera lapinised virus vaccine (HCLV), C-strain, in six-week-old persistently infected pigs following
post-natal infection. CSFV-negative pigs were vaccinated as controls. The humoral and interferon gamma responses as well as the CSFV RNA loads were monitored for 21 days post-vaccination. No vaccine viral RNA was detected in the serum samples and tonsils from CSFV postnatally persistently infected pigs for 21 days post-vaccination. Furthermore, no E2-specific antibody response or neutralising antibody titres were shown in CSFV persistently infected vaccinated animals. Likewise, no of IFN-gamma producing cell response against CSFV or PHA was observed. To our knowledge, this is the first report demonstrating the absence of a response to vaccination in CSFV persistently infected pigs.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
9
Publisher
BMC
Is part of
Veterinary Research
Citation
Muñoz-González, Sara, Marta Perez-Simó, Marta Muñoz, José Alejandro Bohorquez, Rosa Rosell, Artur Summerfield, Mariano Domingo, Nicolas Ruggli, and Llilianne Ganges. 2015. "Efficacy Of A Live Attenuated Vaccine In Classical Swine Fever Virus Postnatally Persistently Infected Pigs". Veterinary Research 46 (1). doi:10.1186/s13567-015-0209-9.
Grant agreement number
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-38343/ES/ESTUDIOS DE INMUNOPATOGENICIDAD FRENTE AL VIRUS DE LA PESTE PORCINA CLASICA (VPPC): IMPLICACIONES PARA EL DESARROLLO DE NUEVAS VACUNAS Y HERRAMIENTAS DIAGNOSTICAS/
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/