High-density mapping suggests cytoplasmic male sterility with two restorer genes in almond x peach progenies
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Author
Donoso, José Manuel
Picañol, Roger
Aranzana, María José
Arús, Pere
Publication date
2015-05-06ISSN
2052-7276
Abstract
Peach (Prunus persica) and almond (Prunus dulcis) are two sexually compatible species that produce fertile offspring. Almond, a highly
polymorphic species, is a potential source of new genes for peach that has a strongly eroded gene pool. Here we describe the genetics
of a male sterile phenotype that segregated in two almond (‘Texas’) 3 peach (‘Earlygold’) progenies: an F2 (T3E) and a backcross one
(T1E) to the ‘Earlygold’ parent. High-density maps were developed using a 9k peach SNP chip and 135 simple-sequence repeats. Three
highly syntenic and collinear maps were obtained: one for the F2 (T3E) and two for the backcross, T1E (for the hybrid) and E (for
‘Earlygold’). A major reduction of recombination was observed in the interspecific maps (T3E and T1E) compared to the intraspecific parent (E). The E map also had extensive monomorphic genomic regions suggesting the presence of large DNA fragments identical by descent. Our data for the male sterility character were consistent with the existence of cytoplasmic male sterility, where individuals having the almond cytoplasm required the almond allele in at least one of two independent restorer genes, Rf1 and Rf2, to be fertile. The restorer genes were located in a 3.4 Mbp fragment of linkage group 2 (Rf1) and 1.4 Mbp of linkage group 6 (Rf2). Both fragments contained several genes coding for pentatricopeptide proteins, demonstrated to be responsible for restoring fertility in other species. The implications of these results for using almond as a source of novel variability in peach are discussed.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
633 - Field crops and their production
Pages
8
Publisher
Oxford University Press
Is part of
Horticulture Research
Citation
Donoso, José Manuel, Iban Eduardo, Roger Picañol, Ignasi Batlle, Werner Howad, María José Aranzana, and Pere Arús. 2015. "High-Density Mapping Suggests Cytoplasmic Male Sterility With Two Restorer Genes In Almond × Peach Progenies". Horticulture Research 2 (1). doi:10.1038/hortres.2015.16.
Grant agreement number
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-40228-C02-01/ES/USO DE LA SECUENCIA GENOMICA PARA LA CARACTERIZACION DE LA VARIABILIDAD INTRACLONAL E INTERESPECIFICA EN MELOCOTONERO Y ALMENDRO/
EC/FP7/265582/EU/Integrated approach for increasing breeding efficiency in fruit tree crops/FRUIT BREEDOMICS
Program
Genòmica i Biotecnologia
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/