Morphological and Molecular Characterization of Dietary-Induced Pseudo-Albinism during Post-Embryonic Development of Solea senegalensis (Kaup, 1858)
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Author
Boglino, Anaïs
Rotllant, Josep
Cerdá-Reverter, José Miguel
Publication date
2013-07-16ISSN
1932-6203
Abstract
The appearance of the pseudo-albino phenotype was investigated in developing Senegalese sole (Solea senegalensis, Kaup
1858) larvae at morphological and molecular levels. In order to induce the development of pseudo-albinos, Senegalese sole
larvae were fed Artemia enriched with high levels of arachidonic acid (ARA). The development of their skin pigmentation
was compared to that of a control group fed Artemia enriched with a reference commercial product. The relative amount of
skin melanophores, xanthophores and iridophores revealed that larval pigmentation developed similarly in both groups.
However, results from different relative proportions, allocation patterns, shapes and sizes of skin chromatophores revealed
changes in the pigmentation pattern between ARA and control groups from 33 days post hatching onwards. The new
populations of chromatophores that should appear at post-metamorphosis were not formed in the ARA group. Further,
spatial patterns of distribution between the already present larval xanthophores and melanophores were suggestive of
short-range interaction that seemed to be implicated in the degradation of these chromatophores, leading to the
appearance of the pseudo-albino phenotype. The expression profile of several key pigmentation-related genes revealed
that melanophore development was promoted in pseudo-albinos without a sufficient degree of terminal differentiation,
thus preventing melanogenesis. Present results suggest the potential roles of asip1 and slc24a5 genes on the downregulation of trp1 expression, leading to defects in melanin production. Moreover, gene expression data supports the
involvement of pax3, mitf and asip1 genes in the developmental disruption of the new post-metamorphic populations of
melanophores, xanthophores and iridophores.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
639 - Hunting. Fishing. Fish breeding
Pages
16
Publisher
Public Library of Science
Is part of
PLoS ONE
Citation
Darias, Maria J., Karl B. Andree, Anaïs Boglino, Josep Rotllant, José Miguel Cerdá-Reverter, Alicia Estévez, and Enric Gisbert. 2013. "Morphological And Molecular Characterization Of Dietary-Induced Pseudo-Albinism During Post-Embryonic Development Of Solea Senegalensis (Kaup, 1858)". Plos ONE 8 (7): e68844. doi:10.1371/journal.pone.0068844.
Grant agreement number
MICINN/Programa Nacional de Proyectos de Investigación Fundamental/AGL2008-03897-C04-01/ES/EL PAPEL DE LOS LIPIDOS SOBRE LA CALIDAD DE LARVAS DE PECES: EFECTOS SOBRE DEFORMACIONES ESQUELETICAS Y PROBLEMAS PIGMENARIOS/ACU
MICINN/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/FJC/ES/ /
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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