Insect glycerol transporters evolved by functional co-option and gene replacement
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Publication date
2015-07-17ISSN
2041-1723
Abstract
Transmembrane glycerol transport is typically facilitated by aquaglyceroporins in Prokaryota and Eukaryota. In holometabolan insects however, aquaglyceroporins are absent, yet several species possess polyol permeable aquaporins. It thus remains unknown how glycerol transport evolved in the Holometabola. By combining phylogenetic and functional studies, here we show that a more efficient form of glycerol transporter related to the water-selective channel AQP4 specifically evolved and multiplied in the insect lineage, resulting in the replacement of the ancestral branch of aquaglyceroporins in holometabolan insects. To recapitulate this evolutionary process, we generate specific mutants in distantly related insect aquaporins and human AQP4 and show that a single mutation in the selectivity filter converted a water-selective channel into a glycerol transporter at the root of the crown clade of
hexapod insects. Integration of phanerozoic climate models suggests that these events were associated with the emergence of complete metamorphosis and the unparalleled radiation of insects.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
639 - Hunting. Fishing. Fish breeding
Pages
7
Publisher
Nature Research
Is part of
Nature Communications
Citation
Finn, Roderick Nigel, François Chauvigné, Jon Anders Stavang, Xavier Belles, and Joan Cerdà. 2015. "Insect Glycerol Transporters Evolved By Functional Co-Option And Gene Replacement". Nature Communications 6 (1). doi:10.1038/ncomms8814.
Grant agreement number
FEDER/ / /EU/ /
MINECO/Programa Estatal de fomento de la investigación científica y técnica de excelencia/AGL2013-41196-R/ES/DESARROLLO DE MARCADORES MOLECULARES DE CALIDAD DE GAMETOS BASADOS EN AQUAPORINAS PARA LA ACUICULTURA DE PECES MARINOS/
MINECO/Programa Nacional de Proyectos de Investigación Fundamental/CGL2012-36251/ES/LA REGULACION DE LA MORFOGENESIS DE LA GLANDULA TERGAL DE LAS CUCARACHAS, COMO MODELO MINIMO DE METAMORFOSIS DE INSECTOS/
MINECO/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/AGL2013-41196-R/ES/Desarrollo de marcadores moleculares de calidad de gametos basados en aquaporinas para la acuicultura de peces marinos/
Program
Aqüicultura
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/