Enhanced antiviral immunity and dampened inflammation in llama lymph nodes upon MERS-CoV sensing: bridging innate and adaptive cellular immune responses in camelid reservoirs
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Publication date
2023-06-14ISSN
1664-3224
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infection can cause fatal pulmonary inflammatory disease in humans. Contrarily, camelids and bats are the main reservoir hosts, tolerant for MERS-CoV replication without suffering clinical disease. Here, we isolated cervical lymph node (LN) cells from MERS-CoV convalescent llamas and pulsed them with two different viral strains (clades B and C). Viral replication was not supported in LN, but a cellular immune response was mounted. Reminiscent Th1 responses (IFN-γ, IL-2, IL-12) were elicited upon MERS-CoV sensing, accompanied by a marked and transient peak of antiviral responses (type I IFNs, IFN-λ3, ISGs, PRRs and TFs). Importantly, expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8) or inflammasome components (NLRP3, CASP1, PYCARD) was dampened. The role of IFN-λ3 to counterbalance inflammatory processes and bridge innate and adaptive immune responses in camelid species is discussed. Our findings shed light into key mechanisms on how reservoir species control MERS-CoV in the absence of clinical disease.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
8
Publisher
Frontiers Media
Is part of
Frontiers in Immunology
Citation
Rodon, Jordi, Nigeer Te, Joaquim Segalés, Júlia Vergara-Alert, and Albert Bensaid. 2023. "Enhanced Antiviral Immunity And Dampened Inflammation In Llama Lymph Nodes Upon MERS-Cov Sensing: Bridging Innate And Adaptive Cellular Immune Responses In Camelid Reservoirs". Frontiers In Immunology 14. doi:10.3389/fimmu.2023.1205080.
Grant agreement number
EC/PF7/115760/EU/Zoonotic Anticipation and Preparedness Initiative/ZAPI
EC/H2020/731014/EU/Veterinary Biocontained facility Network for excellence in animal infectiology research and experimentation /VetBioNet
Program
Sanitat Animal
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2555]
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Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/