Protection provided by a commercial modified-live porcine reproductive and respiratory syndrome virus (PRRSV) 1 vaccine (PRRSV1-MLV) against a Japanese PRRSV2 field strain
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Author
Miranda, Joel
Romero, Salvador
de Lucas, Lidia
Saito, Fumitoshi
Fenech, Mar
Díaz, Ivan
Publication date
2023-07-07ISSN
1229-845X
Abstract
Background: Porcine reproductive and respiratory syndrome virus (PRRSV) vaccines do not provide full cross-protection, mainly due to the virus genetic variability. Despite this, vaccines based on modified-live PRRSV (PRRSV-MLV) reduce the disease impact.
Objectives: To assess the efficacy of two commercial vaccines-one based on PRRSV1 (PRRSV1-MLV) and another on PRRSV2 (PRRSV2-MLV)-against a Japanese PRRSV2 field strain.
Methods: Two groups of three-week-old piglets were vaccinated (G1: PRRSV1-MLV; G2: PRRSV2-MLV) and two were kept as non-vaccinated (INF and CTRL). One month later, G1, G2, and INF were challenged with a PRRSV2 field strain.
Results: After the challenge, clinical signs were only observed in INF. Moreover, the highest rectal temperatures and values for the area under the curve (AUC) were observed in INF. Regarding viral detection, both AUC and the proportion of positive samples in blood were higher in INF. In G1, viremic animals never reached 100%. At necropsy (21 d after the challenge), differences for titers among groups were only found in tonsils (G1 < G2 and INF). One animal (belonging to G1) was negative in all tissues. Regarding humoral responses, G1 and G2 seroconverted after vaccination, as detected in the corresponding enzyme-linked immunosorbent assay. Specific neutralizing antibodies (NA) against PRRSV1-MLV were already detected at 14 d after vaccination in G1, showing a significant booster after the challenge, while PRRSV2-MLV NA were detected in G2 at the end of the experiment.
Conclusions: Despite genetic differences, PRRSV1-MLV has been demonstrated to confer partial protection against a Japanese PRRSV2 strain, at least as good as PRRSV2-MLV.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
619 - Veterinary science
Pages
13
Publisher
Korean Society of Veterinary Science
Is part of
Journal of Veterinary Science
Citation
Miranda, Joel, Salvador Romero, Lidia de Lucas, Fumitoshi Saito, Mar Fenech, and Ivan Díaz. 2023. "Protection Provided By A Commercial Modified-Live Porcine Reproductive And Respiratory Syndrome Virus (PRRSV) 1 Vaccine (PRRSV1-MLV) Against A Japanese PRRSV2 Field Strain". Journal Of Veterinary Science 24. doi:10.4142/jvs.23025.
Program
Sanitat Animal
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- ARTICLES CIENTÍFICS [2555]
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