Germination of Pisum sativum L. Seeds Is Associated with the Alternative Respiratory Pathway
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Author
Rodrigues, Lénia
Nunes, João
Rodrigues, Leonardo
Hansen, Lee D.
Cardoso, Hélia
Publication date
2023-10-09ISSN
2079-7737
Abstract
Germination is a complex process that begins with rapid water uptake and a marked increase in oxygen consumption rate from initiation of aerobic respiration. The mitochondrial electron transport chain (ETC) in plants is a critical cellular respiratory system that has two different pathways: the cytochrome oxidase (COX) pathway and the alternative oxidase (AOX) pathway. The AOX enzyme plays a crucial role in energy production as well as in stress response. An AOX gene family study in Pisum sativum revealed three AOX genes (PsAOX). Transcript accumulation analysis revealed the involvement of all three PsAOX members in pea seed germination, albeit with high genotype variation. Calorespirometry was used to monitor metabolic changes during a germination trial using inhibitors of both respiratory pathways and proved to be useful for distinguishing differing respiratory activity in different cultivars. This study offers valuable insights into the AOX family composition in P. sativum, as well as their role during seed germination.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
631 - Agriculture in general
Pages
22
Publisher
MDPI
Is part of
Biology
Citation
Rodrigues, Lénia, Amaia Nogales, João Nunes, Leonardo Rodrigues, Lee D. Hansen, and Hélia Cardoso. 2023. “Germination of Pisum Sativum L. Seeds Is Associated with the Alternative Respiratory Pathway.” MDPI. Multidisciplinary Digital Publishing Institute. October 9. doi:10.3390/biology12101318
Grant agreement number
EC/H2020/727230/UE/Improve performance of organic agriculture by boosting organic seed and plant breeding efforts across Europe/LIVESEED
Program
Protecció Vegetal Sostenible
This item appears in the following Collection(s)
- ARTICLES CIENTÍFICS [2845]
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/